Skip to main content

Controlling the bacterial load of Salmonella Typhi in an experimental mouse model by a lytic Salmonella phage STWB21: a phage therapy approach



Salmonella enterica serotype Typhi is one of the major pathogens causing typhoid fever and a public health burden worldwide. Recently, the increasing number of multidrug-resistant strains of Salmonella spp. has made this utmost necessary to consider bacteriophages as a potential alternative to antibiotics for S. Typhi infection treatment. Salmonella phage STWB21, isolated from environmental water, has earlier been reported to be effective as a safe biocontrol agent by our group. In this study, we evaluated the efficacy of phage STWB21 in reducing the burden of salmonellosis in a mammalian host by inhibiting Salmonella Typhi invasion into the liver and spleen tissue.


Phage treatment significantly improved the survival percentage of infected mice. This study also demonstrated that oral administration of phage treatment could be beneficial in both preventive and therapeutic treatment of salmonellosis caused by S. Typhi. Altogether the result showed that the phage treatment could control tissue inflammation in mice before and after Salmonella infection.


To the best of our knowledge, this is the first report of phage therapy in a mouse model against a clinically isolated Salmonella Typhi strain that includes direct visualization of histopathology and ultrathin section microscopy images from the liver and spleen sections.

Peer Review reports


Salmonella enterica, a Gram-negative bacterium that belongs to the Enterobacteriaceae family, is generally divided into typhoidal Salmonella (TS) and non-typhoidal Salmonella (NTS) serotypes based on clinical symptoms [1]. A potentially fatal multisystemic infection, typhoid fever or enteric fever, is primarily produced by the human-adapted pathogen Salmonella enterica serovar Typhi and significantly contributes to global morbidity and mortality in low-and middle-income countries [2]. It is a common foodborne pathogen that is primarily present in poultry, eggs, and dairy products [3]. It may be transferred by human feces, contaminated food, water, and person-to-person contact. The fecal-oral route is the most prevalent way of transmission [4]. The risk of morbidity and death associated with typhoid fever can be decreased by treating the condition with appropriate antibiotics.

During acute infection of S. Typhi shedding, the dosage and duration are important determinants in antibiotic therapy [5]. According to the WHO priority list of pathogens, Salmonella belongs to the highest priority pathogens group that needed attention to search for new antibiotics. Fluoroquinolone, ampicillin, trimethoprim-sulfamethoxazole, and chloramphenicol made up the conventional typhoid fever treatment regimens [6, 7]. Nevertheless, most of the bacterial strains are now found to be resistant due to the development of multidrug-resistance mechanisms. The percentage of typhoid fever isolates that were nalidixic acid-resistant was reported to be over 60% in Kolkata [8]. As a result of this, third-generation cephalosporins are now being used more often. Moreover, first- and second-line antibiotics and both fluoroquinolones and third-generation cephalosporins-resistant Salmonella strains have been reported in Nepal and Eastern India [9,10,11]. The World Health Organization (WHO) has given an estimate that by 2050 drug-resistant infections will kill millions of people every year [12]. The increase in treatment cost due to the unavailability of a suitable antibiotic will create a huge economic burden and may push millions of people into acute poverty [13]. This is definitely minacious to mankind and requires to be handled with an unconventional yet affordable treatment strategy such as phage therapy [9, 14].

Bacteriophages are bacterial viruses, abundant in nature, highly specific, and effective in killing their targeted host bacteria [15]. The mechanism of killing bacteria and developing bacterial resistance in the case of phage and antibiotics is fundamentally different [16,17,18]. The advantage of phage usage in the prevention of bacterial infections is due to their strict host specificity, high bactericidal efficiency, safety, low cost of production, and ease of extraction and preservation [19, 20]. However, the therapeutic efficacy of phage treatment has been explicitly demonstrated by only a handful of successful clinical trials that adhered to the current norms of evidence-based medicine [21]. Therefore, to conduct a trial with a greater possibility of success, it is crucial to analyse carefully the data obtained from experimental trials in animals.

In vivo investigation using laboratory animals is an essential component of research on determining the efficacy and safety of novel isolated bacteriophages [22]. An accurate assessment of the mechanism of phage therapy in living organisms can be preferred using animal models such as mouse, rat, and rabbit models because of their genetic closeness to humans. These models also provide information on the immune response (including any potential interactions with immune system cells like phagocytes), the gut microbiota, and infected tissue, as well as the extent of safety, tolerability, and observation of any possible adverse effects of the preparation being used [23, 24]. Our aim was to evaluate the efficacy of phage STWB21 in an animal model that would be advantageous in the future for the treatment of illnesses brought on by the Salmonella Typhi bacterium. In our previous work, we have shown Salmonella phage STWB21 fulfils the initial criteria to be a potential antibacterial agent [25]. In this study, we report that the phage STWB21 can control Salmonella invasion both prophylactically and therapeutically in mice. Additionally, we compared the preventive and therapeutic benefits of STWB21 treatment on mice against lethal S. Typhi infection without producing any negative effects, emphasizing the clinical importance of this phage to S. Typhi-induced illnesses.


Phage propagation

After overnight incubation, the plaques of phage on the HEA plate with S. Typhi bacterial lawn were measured around 1 mm in diameter. It indicates the presence of S. Typhi-specific phage STWB21 (Fig. 1).

Fig. 1
figure 1

Plaques of STWB21 against S. Typhi on HEA plate

Determination of LD50

All infected mice in the LD50 determination study developed illness starting at the post-challenge. Only a few mice in groups 1, 2, and 3 were still sick at the end of the experiment, and death was nil or minimal. However, significant deaths occurred in groups 4 and 5. From the post-challenge day onwards, these mice began to perish, and by the conclusion of the trial, every mouse that had survived was gravely sick. Figure 2 displays the mortality of the mice on the fifth day following infection. The mean fatal dosage was determined to be 3 × 107 CFU/mice based on the observation.

Fig. 2
figure 2

Determination of the mean lethal dose (LD50) of S. Typhi host strain in BALB/c mice n = 6; each group was intraperitoneally (i.p) infected with serially diluted bacterial suspensions of S. Typhi. The percentage of survival was determined for 7 days

Oral treatment with phage STWB21 significantly reduced Salmonella proliferation in mice

The significant effectiveness of phage STWB21 against the S. Typhi infection was examined in a mouse model. The effect of phage indicated decreased colonization (Fig. 3A) in both the prevention group (or prophylactic group) and the treatment group (or therapeutic group) as shown in Figs. 3B and  3C. Additionally, after observing 4 days, the survival rate of the prevention group is almost 66% (Fig. 3D) in comparison with the treated group which is almost 33% (Fig. 3E). However, the viability of the phage control group was similar to the PBS control group, indicating the safety of phage STWB21.

Fig. 3
figure 3

(A) Salmonella bacterial colonization on HEA plate. (B) Colonization of prophylactic treatment. (C) Colonization of therapeutic treatment. (D) Impact of single dose phage treatment on S. Typhi before infection (prophylactic treatment) and survival rates of BALB/c mice. (E) Impact of single dose phage treatment on S. Typhi after infection (therapeutic treatment) and survival rates of BALB/c mice. A statistically significant difference (*P < 0.05) was observed using 6 mice per group on phage treatment. Error bars represent the standard error of the mean (SEM) of three independent replicates and data were analyzed using GraphPad Prism 5.0

Comparison of preventive versus therapeutic phage treatment using light microscopy and transmission electron microscopy

A comparative study was carried out to evaluate the effectiveness of phage STWB21 treatment prophylactically and therapeutically. Figures 4 and 5, showed the light microscopy images of histopathological sections and electron microscopic images of the stained ultrathin sections of mice liver tissue respectively. In Fig. 4A, the liver section of normal mice showed normal histology with central vein and hepatocytes arranged in the hepatic cords, and in Fig. 5A, a round nucleus (NU), normal mitochondria (MT), and endoplasmic reticulum (ER) were observed. However, in infected mice, pyknotic nuclei (PN) with the appeared nucleolus, mitochondria (M), and fragmented rough endoplasmic reticulum (RER), liver necrosis, and abscess were observed with hepatomegaly in Figs. 4B and 5B. Transmission electron micrographs of both the preventive group and therapeutic group (Fig. 5C and 5D) showed an elevation in lysosomes and mitochondria level and an increase in their density too. Interestingly, the preventive group showed minimal damage rather than the therapeutic group.

Fig. 4
figure 4

 H&E staining sections of the liver from normal, infected, and treated mice. Sections were stained with hematoxylin and eosin. Magnification, X10. (A) Normal: without any infection or treatment. Central venule, hepatic sinusoids, plates of hepatic cells, and portal areas were seen. (B) Infected: mice liver at 48 h post-challenge. The central venule was inflamed. (C) Liver histopathological sections of Prophylactic treatment grouped mice. (D) Liver histopathological sections of Therapeutic treatment grouped mice

Fig. 5
figure 5

Transmission electron micrograph of mice liver sections. Magnification, X 1500. (A) Control: mouse liver section showing normal hepatic architecture (M: Mitochondria, N: Nucleus, L: Lysosome, ER: Endoplasmic reticulum). (B) Infected: mice liver at 48 h post-challenge. The central venule was inflamed. (C) Prophylactic treatment grouped mice. (D) Therapeutic treatment grouped mice

The spleen is a large secondary lymphoid organ and it is composed of two compartments: red pulp and white pulp. Figure 6 showed the histopathological sections of the spleen tissue. A clear distinction between the red and white pulp, lymphoid follicles, and marginal zones was observed in the spleen of normal mice in Figs. 6A and 7A.

Fig. 6
figure 6

Images from the light microscope of mice spleen histopathological sections stained with hematoxylin and eosin. Magnification, X10. (A) Control: without any treatment. Red pulp, white pulp, plates of hepatic cells, and portal areas were visible. (B) Infected: mice spleen at 48 h post-challenge. The central venule was inflamed. (C) Spleen histopathological sections of preventive grouped mice. (D) Spleen histopathological sections of therapeutic treatment grouped mice

Fig. 7
figure 7

Transmission electron micrograph of mice spleen sections. Magnification, X 1500. (A) Control: without any treatment. Central venule, hepatic sinusoids, plates of hepatic cells, and portal areas were seen. (B) Infected: mice liver at 48 h post-challenge. The central venule was inflamed. (C) Prophylactic treatment grouped mice. (D) Therapeutic treatment grouped mice

However, the S. Typhi-infected spleen in Figs. 6B and 7B showed severe congestion and enlarged red pulp, splenomegaly, inflamed central artery, lymphoid follicle hyperplasia, and eventually disruption of white pulp structure. But in the preventive group (Figs. 6C and 7C) and therapeutic group (Figs. 6D and 7D), significant changes were observed. In the therapeutic group, the white pulp structure was eventually disrupted but not as severe as in the infected group. In contrast, the preventive group showed less structural disorganization than the therapeutic group.


S. enterica contains over 2500 identified serotypes, and they differ significantly in terms of pathogenicity. Among them, S. Typhi is one of the major Salmonella enterica serovars that cause enteric fever or salmonellosis in humans. Additionally, it has the ability to produce biofilm, which is pathogenic in nature and also develops antibiotic-resistant bacteria. Antimicrobial medicines are mostly used to treat and prevent salmonellosis in humans and animals, they are nevertheless employed in certain nations to encourage the development of food animals [26]. In hospitals, communities, and livestock settings, their indiscriminate usage has facilitated the development of bacterial resistance. Either through the expansion of the resistant bacteria or through the transfer of resistance genes from animal to human, AMR can spread from animals to humans and vice versa [27]. Additionally, the development and spread of multidrug-resistant bacteria have provided a strong motivation to explore effective prophylactic and therapeutic means of eradication of bacterial infections. In this regard, the concept of phage therapy for controlling bacterial infections has been widely accepted.

With a population estimated to be > 1030, phages are the most prevalent biological species on earth [28]. Because of this, they can be investigated using several bacterial models and infect a wide range of bacterial species. For therapeutic purposes, tailed bacteriophages (dsDNA viruses) are recommended and they belong to a single order of Caudovirales. They typically contain a tail with or without tail fibers and a head or capsid with a dsDNA. During bacterial attachment, the phage uses its tail, tail fibers, or both to create precise interactions with the surface [29].

Studies from previous groups that used BALB/c mice as an infection model and oral administration of phages for therapy showed promising outcomes and phages were shown to cure bacterial infections more effectively [30]. Based on other reported studies, the phage STWB21 was also administered orally along with PBS buffer. A single dose of phage STWB21 at MOI 1 was given 60 min before and after infecting mice with S. Typhi bacteria resulting in the rescue of 66% and 33% of the mice, respectively. This was a remarkable result compared to the control group, which caused the death of all infected mice. The effect of phage STWB21 only and the PBS buffer showed no side effects on the health of the experimental mice groups. Thus, phage rescue experiments could be conducted without bias.

In this study, the most important finding was that the use of phage STWB21 has reduced the number of Salmonella Typhi colonies in mice in preventive as well as therapeutic treatment. These findings further suggest that phage STWB21 can both reduce the adversity of salmonellosis and control the transmission of the bacteria to the environment.

Previous studies from other groups reported that prophylactic bacteriophage administration is more effective than therapeutic administration in phage therapy using a single phage or phage cocktail [31, 32]. The current study also showed that the prophylactic administration of phage STWB21 decreases the colonization of a foodborne intestinal pathogen S. Typhi and represents a potential strategy to reduce the spreading of Salmonella Typhi during the outbreak.

In our previous study, we reported a T5-like phage STWB21, a member of the Siphoviridae family with a dsDNA of length 112,834 bp and a 40.37% GC content [25]. The phage STWB21 against pathogenic S. Typhi was found to successfully eliminate or reduce the bacteria in vitro. Here we are reporting the in vivo laboratory animal studies on the evaluation of the safety and effectiveness of this phage. BALB/c mice model system revealed promising results to be considered as important information for future clinical trials. Relentless efforts are being taken to use phage therapy during the post-antibiotic era and the current study is one such attempt for a better understanding of the usage of lytic phages as a therapeutic tool. However, the results of this study demonstrate that oral phage administration is significantly efficacious in the treatment of salmonellosis and other infections caused by S. Typhi.

Materials and methods

Bacterial strains and culture conditions

A clinically isolated Salmonella Typhi (Kol-551) strain was obtained from the Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases. The strain was preserved at − 80 °C in 8% glycerol-containing brain heart infusion broth (BHIB; BD Difco). The bacteria were grown on tryptic soy agar (TSA; BD Difco) and tryptic soy broth (TSB; BD Difco) at 37 °C.

Phage preparation

Salmonella phage STWB21 isolated from the lake water of an outbreak area about 18 km from Kolkata, West Bengal, India. The phage sample was prepared by a double-layer agar method as previously described [25, 33]. Briefly, 250 µl of mid-log (exponential) phase liquid culture of S. Typhi was mixed with phage at a multiplicity of infection (MOI) of 0.01 and plated on soft agar (0.8%) overlay (3 ml) on nutrient agar and incubated at 37 °C. After that, the soft agar layer was scraped off when complete lysis occurred and suspended in 1 ml of Tris–MgCl2 buffer followed by centrifugation at 10,000 × g for 20 min at 4°C [34]. Then, the supernatant was purified and concentrated by ultracentrifugation (25,000 rpm, 1.5 h, 4 °C) and sucrose step-gradient ultracentrifugation (30,000 rpm, 2 h, 4 °C) respectively [35]. The concentrated phage STWB21 was preserved at 4 °C.

Animal experiment

Selection of animals

BALB/c mice were collected from the NICED animal house facility to investigate the effect of phage STWB21 on Salmonella Typhi. Six weeks old, disease-free, healthy, active female and male BALB/c mice were chosen for the experimental purpose with animals weighing in the range of 22 ± 2 gm. Mice were fed sterile food and water ad libitum. Animals were kept for 10 days before experimentation to acclimatize to laboratory conditions. The animals were housed and the entire experiment was carried out in the animal house of ICMR-NICED.

Determination of LD50

To determine the LD50 of Salmonella Typhi (Kol-551), a total of thirty mice were distributed into five groups of six mice in each group and intraperitoneally injected with different Salmonella doses (from 1 × 104 to 3 × 107 colony-forming units/group). Six mice per group were kept in cages and challenged with above-mentioned inoculum of the Salmonella strain in respective groups. Mice were closely monitored and deaths of mice were noted during 7 days. The experiment was done in triplicates.

Efficacy of bacteriophage STWB21 in challenged BALB/c mice

To compare the efficiency of prophylactic versus therapeutic application of phage STWB21 on S. Typhi shedding, BALB/c mice were divided into five groups and each group consisted of 6 animals. The doses were fixed and prepared in PBS and administered. Mice from Group I received only PBS as a control, Group II animals were administered by intraperitoneal injection (i.p.) with S. Typhi (4.5 × 108 CFU) diluted in PBS. Group III animals were orally administered with only STWB21 phage (1.5 × 1010 PFU) to check any lethal effect of phage on mice. Group IV mice represent the prevention group and Group V mice represent the treatment group. All five groups were kept in hygienic conditions with a continuous supply of food and water for 14 days. A significant observation was made and results were recorded (Table 1).

Table 1 Group-wise distribution of mice with intraperitoneal administration of bacteria and oral administration of phage

Enumerations of Salmonella and phages from mice tissues

Mice tissues (liver, and spleen) were collected on day two post-infection when the most prominent gut inflammation occurred [36]. Tissues were immediately weighed and homogenized using a bead-beating machine with 1.0 mm diameter silica beads (Biospec Products, Bartlesville, United States). Salmonella Typhi colony-forming unit (CFU)/gm tissues were determined by a serial dilution technique on the HEA agar.

H&E staining

At indicated time points, mice were euthanized and intestinal organs were carefully removed and fixed with 10% buffered formalin and embedded in paraffin. Six-micrometer sections were cut and stained with hematoxylin and eosin [37]. The stained sections were observed under a JENOPTIK GRYPHAX digital microscope.

Preparation and imaging of tissue sections by electron microscopy

For transmission electron microscopy (TEM) analysis, liver and spleen tissues were cut into 1 mm x 1 mm pieces and further processed following the previously described method [38]. Tissues were fixed in cacodylate-buffered glutaraldehyde at 4 °C overnight. After that, tissues were then post-fixed in 1% osmium tetroxide followed by dehydration with a graded series of ethanol (25%, 50%, 75%, and 100%). Then the samples were embedded in resin Agar100 and polymerization was done overnight at 60 °C. Gold sections of 60–90 nm were cut using a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Germany) fitted with a Diatome diamond knife (Diatome, Hatfield, PA). Then the sections were transferred to 200 mesh copper or nickel grids (EMS). Next, the sections were dual-stained with 2% aqueous uranyl acetate and 0.2% lead citrate, and grids were air-dried. Grids were imaged using a JEOL 1400 electron microscope at an accelerating voltage of 120 kV.

Statistical analyses

Data were expressed as mean ± standard deviation (SD). Three technical and three biological repeats were carried out, as needed. GraphPad Prism version 5 was used to carry out all the statistical analyses. Statistical significance was assessed using two-way ANOVA (*P < 0.05; **P < 0.01).


In our previously published research, we found that the phage STWB21 against pathogenic S. Typhi successfully eliminated or reduced the bacterial population in vitro. These findings led us to undertake studies on the impact of the phage STWB21 in vivo. The results of the current investigation showed that phage STWB21 can prevent and treat Salmonella invasion in mouse models. The clinical significance of this phage to control S. Typhi-induced illnesses is further supported by the possibility of the oral administration of phage treatment. It may be advantageous in both preventive and therapeutic treatment of salmonellosis caused by S. Typhi without developing any adverse effects.

Data Availability

The dataset used and/or analyzed during the current study is added in the manuscript. Additional datasets are not available from the authors.



Luria Bertani broth


Multiplicity of infection


Hektoen enteric agar


Transmission electron microscopy


Colony-forming units


Plaque-forming units


Phosphate-buffered saline


  1. Feasey NA, Dougan G, Kingsley RA, Heyderman RS, Gordon MA. Invasive non-typhoidal salmonella disease: an emerging and neglected tropical disease in Africa. Lancet. 2012;379(9835):2489–99.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Als D, Radhakrishnan A, Arora P, et al. Global Trends in Typhoidal Salmonellosis: a systematic review. Am J Trop Med Hyg. 2018;99(3Suppl):10–9.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Silva CA, Blondel CJ, Quezada CP, Porwollik S, Andrews-Polymenis HL, Toro CS, Zaldívar M, Contreras I, McClelland M, Santiviago CA. Infection of mice by Salmonella enterica serovar Enteritidis involves additional genes that are absent in the genome of serovar typhimurium. Infect Immun. 2012;80(2):839–49.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Mogasale VV, Ramani E, Mogasale V, Park JY, Wierzba TF. Estimating typhoid fever risk associated with lack of access to safe water: a systematic literature review. J Environ Public Health 2018; 2018:9589208.

  5. Gibani MM, Voysey M, Jin C, et al. The impact of vaccination and prior exposure on stool shedding of Salmonella typhi and Salmonella paratyphi in 6 controlled human infection studies. Clin Infect Dis. 2019;68:1265–73.

    Article  CAS  PubMed  Google Scholar 

  6. Crump JA, Medalla FM, Joyce KW, et al. Antimicrobial resistance among invasive nontyphoidal Salmonella enterica isolates in the United States: National Antimicrobial Resistance Monitoring System, 1996 to 2007. Antimicrob Agents Chemother. 2011;55(3):1148–54.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Sjölund-Karlsson M, Joyce K, Blickenstaff K, et al. Antimicrobial susceptibility to azithromycin among Salmonella enterica isolates from the United States. Antimicrob Agents Chemother. 2011;55(9):3985–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Azmatullah A, Qamar FN, Thaver D, Zaidi AK, Bhutta ZA. Systematic review of the global epidemiology, clinical and laboratory profile of enteric fever. J Glob Health. 2015;5(2):020407.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Shrestha KL, Pant ND, Bhandari R, Khatri S, Shrestha B, Lekhak B. Re-emergence of the susceptibility of the Salmonella spp. isolated from blood samples to conventional first line antibiotics. Antimicrob Resist Infect Control. 2016;5:22.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Hendriksen RS, Vieira AR, Karlsmose S, et al. Foodborne Pathog Dis. 2011;8(8):887–900. Global monitoring of Salmonella serovar distribution from the World Health Organization Global Foodborne Infections Network Country Data Bank: results of quality assured laboratories from 2001 to 2007.

  11. Samajpati S, Pragasam AK, Mandal S, Balaji V, Dutta S. Emergence of ceftriaxone resistant Salmonella enterica serovar Typhi in Eastern India. Infect Genet Evol. 2021;96:105093.

    Article  CAS  PubMed  Google Scholar 

  12. de Kraker ME, Stewardson AJ, Harbarth S. Will 10 million people die a year due to Antimicrobial Resistance by 2050? PLoS Med. 2016;13(11):e1002184.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Zhen X, Lundborg CS, Sun X, Hu X, Dong H. Economic burden of antibiotic resistance in ESKAPE organisms: a systematic review. Antimicrob Resist Infect Control. 2019;8:137.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Wain J, Kidgell C. The emergence of multidrug resistance to antimicrobial agents for the treatment of typhoid fever. Tans R Soc Trop Med Hyg. 2004;98:423–30.

    Article  CAS  Google Scholar 

  15. Fernández L, Gutiérrez D, García P, Rodríguez A. The perfect bacteriophage for therapeutic Applications-A Quick Guide. Antibiot (Basel). 2019;8(3):126.

    Article  CAS  Google Scholar 

  16. Sulakvelidze A, Alavidze Z, Morris JG. Jr. Bacteriophage therapy. Antimicrob Agents Chemother. 2001;45:649–59.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Lu TK, Koeris MS. The next generation of bacteriophage therapy. Curr Opin Microbiol. 2011;14:524–31.

    Article  PubMed  Google Scholar 

  18. Merril CR, Scholl D, Adhya SL. The prospect for bacteriophage therapy in western medicine. Nat Rev Drug Discov. 2003;2:489–97.

    Article  CAS  PubMed  Google Scholar 

  19. Miedzybrodzki R, Switala-Jelen K, Fortuna W, et al. Bacteriophage preparation inhibition of reactive oxygen species generation by endotoxin-stimulated polymorphonuclear leukocytes. Virus Res. 2008;131(2):233–42.

    Article  CAS  PubMed  Google Scholar 

  20. Haq IU, Chaudhry WN, Akhtar MN, et al. Bacteriophages and their implications on future biotechnology: a review. Virol J. 2012;9:9.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Schooley RT, Biswas B, Gill JJ et al. Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection [published correction appears in Antimicrob Agents Chemother. 2018;62(12):]. Antimicrob Agents Chemother. 2017;61(10):e00954-17. Published 2017 Sep 22.

  22. Cieślik M, Bagińska N, Górski A, Jończyk-Matysiak E. Animal Models in the Evaluation of the Effectiveness of Phage Therapy for Infections Caused by Gram-Negative Bacteria from the ESKAPE Group and the Reliability of Its Use in Humans. Microorganisms. 2021;9(2):206. Published 2021 Jan 20.

  23. Hsu BB, Gibson TE, Yeliseyev V, et al. Dynamic modulation of the Gut Microbiota and Metabolome by Bacteriophages in a mouse model. Cell Host Microbe. 2019;25(6):803–814e5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Brix A, Cafora M, Aureli M, Pistocchi A. Animal models to translate phage therapy to Human Medicine. Int J Mol Sci. 2020;21(10):3715.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Mondal P, Mallick B, Dutta M, Dutta S. Isolation, characterization, and application of a novel polyvalent lytic phage STWB21 against typhoidal and nontyphoidal Salmonella spp. Front Microbiol. 2022;13:980025. Published 2022 Aug 22.

  26. Woolhouse M, Ward M, van Bunnik B, Farrar J. Antimicrobial resistance in humans, livestock and the wider environment. Philos Trans R Soc Lond B Biol Sci. 2015;370(1670):20140083.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Argudín MA, Deplano A, Meghraoui A, et al. Bacteria from animals as a Pool of Antimicrobial Resistance genes. Antibiot (Basel). 2017;6(2):12.

    Article  CAS  Google Scholar 

  28. Hendrix RW. Bacteriophages: evolution of the majority. Theor Popul Biol. 2002;61:471–80.

    Article  PubMed  Google Scholar 

  29. Mehmood Khan F, Manohar P, Singh Gondil V, et al. The applications of animal models in phage therapy: an update. Hum Vaccin Immunother. 2023;19(1):2175519.

    Article  PubMed  PubMed Central  Google Scholar 

  30. Kumar RN, Surekha MV, Ramalingam B, et al. Oral toxicity study for Salmonella Killing Lytic Bacteriophage NINP13076 in BALB/c mice and its Effect on Probiotic Microbiota. Curr Microbiol. 2022;79(3):89.

    Article  CAS  PubMed  Google Scholar 

  31. Ahmadi M, Karimi Torshizi MA, Rahimi S, Dennehy JJ. Prophylactic bacteriophage Administration more effective than post-infection administration in reducing Salmonella enterica serovar Enteritidis Shedding in Quail. Front Microbiol. 2016;7:1253.

    Article  PubMed  PubMed Central  Google Scholar 

  32. Lamy-Besnier Q, Chaffringeon L, Lourenço M, et al. Prophylactic administration of a bacteriophage cocktail is safe and effective in reducing Salmonella enterica serovar typhimurium burden in vivo. Microbiol Spectr. 2021;9(1):e0049721.

    Article  PubMed  Google Scholar 

  33. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold spring harbor laboratory press; 1989.

  34. Jaiswal A, Koley H, Mitra S, Saha DR, Sarkar B. Comparative analysis of different oral approaches to treat Vibrio cholerae infection in adult mice. Int J Med Microbiol. 2014;304(3–4):422–30.

    Article  CAS  PubMed  Google Scholar 

  35. Dutta M, Ghosh AN. Physicochemical characterization of El Tor vibriophage S20. Intervirology. 2007;50(4):264–72.

    Article  CAS  PubMed  Google Scholar 

  36. Tsolis RM, Xavier MN, Santos RL, Bäumler AJ. How to become a top model: impact of animal experimentation on human Salmonella disease research. Infect Immun. 2011;79(5):1806–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Sarichai P, Buddhasiri S, Walters GE, Khantawa B, Kaewsakhorn T, Chantarasakha K, et al. Pathogenicity of clinical Salmonella enterica serovar typhimurium isolates from Thailand in a mouse colitis model. Microbiol Immunol. 2020;64:679–93.

    Article  CAS  PubMed  Google Scholar 

  38. Mallick B, Mondal P, Dutta M. Morphological, biological, and genomic characterization of a newly isolated lytic phage Sfk20 infecting Shigella flexneri, Shigella sonnei, and Shigella dysenteriae1. Sci Rep. 2021;11(1):19313. Published 2021 Sep 29.

Download references


We thankfully acknowledge Mrs. Arpita Sarbajna and Mr. Bivash Ranjan Mallick from EM Division, ICMR-NICED for their technical assistance in ultramicrotomy and histopathology sample preparations. We gratefully acknowledge Mr. Debanjan Mukherjee (USIC, University of Burdwan) for his technical assistance in TEM imaging. PM, PH, and BM are financially supported by CSIR-Senior Research Fellowship, ICMR- Senior Research Fellowship [ICMR-3/1/3/JRF-2018/HRD-066(66125)], UGC-Senior Research Fellowship, Govt. of India respectively.


This research received no external funding.

Author information

Authors and Affiliations



P.M. and M.D. conceived and designed the work. P.M., P.H., and B.M. performed the experiments. S.D. provided the typhoidal Salmonella strains and reviewed the manuscript. S.B. helped in the TEM imaging experiment of the liver and spleen. P.M. analyzed the data, wrote the main manuscript text, and prepared Figs. 1, 2, 3, 4, 5, 6 and 7. P.H. contributed in preparing Figs. 2 and 3. M.D. and H.K. supervised the work, analyzed data, and edited the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Moumita Dutta.

Ethics declarations

Ethical Approval

All methods were carried out by relevant guidelines and regulations. The animal experiment protocol was reviewed and conducted following the standard operating procedure as outlined by the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment and Forest, Government of India. The Institutional Animal Ethical Committee (IAEC) of NICED approved the animal experimental protocol with Registration No. 68/GO/Rebi/S/1999/ CPCSEA Valid 17/7/2024, Approval No. PRO/186/ - Nov2022-25.

Consent for publication

Not Applicable.

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mondal, P., Halder, P., Mallick, B. et al. Controlling the bacterial load of Salmonella Typhi in an experimental mouse model by a lytic Salmonella phage STWB21: a phage therapy approach. BMC Microbiol 23, 324 (2023).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: