Table 4 Bacterial strains, plasmids, and primers used in this study

 Escherichia coli DH5α

F−, φ 80dlacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rK−, mK+), phoA, supE44, λ−, thi-1, gyrA96, relA1

[49]

 Bacillus subtilis 168

trpC2

[50]

 Lacticaseibacillus rhamnosus GG

ATCC^a 53,103

Purchased from ATCC

 pUB19

E. coli-B. subtilis shuttle vector, Ap^r, Km^r

[51]

 pUB19-cotG-p40

Spore display of p40 using the CotG anchor

This study

 CotG-F

5′-CCCTTCGACGCGT^bCAGCTGGC-3′

This study

 CotG-R

5′-ACTTGTGTCGCTTCCTCCTCCTCCTTTGTATTTC

TTTTTGACTACCCAGCAATTGCCGTC-3′

This study

 p40-F

5′-TACAAAGGAGGAGGAGGAAGCGACACAAGTG

CCAGCATCGCATCTAACAAGAGCG-3′

This study

 p40-R

5′- AAGGAAAAAGCGGCCG^†CAAAAGGAAAATTA

CCGGTGGATGTAAACGTAGCTGCTGGC-3’

This study