Table 2 List of oligonucleotide sequences and cycling conditions used in the study

RD1

AAGCGGTTGCCGCCGACCGACC

CTGGCTATATTCCTGGGCCCGG GAGGCGATCTGGCGGTTTGGGG

Present (146 bp)

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min.

RD4

ATGTGCGAGCTGAGCGATG TGTACTATGCTGACCATGCG AAAGGAGCACCATCGTCCAC

Present (172 bp)

Absent (268 bp)

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min

RD9

CAAGTTGCCGTTTCGAGCC CAATGTTTGTTGCGCTGC GCTACCCTCGACCAAGTGTT

Present (235 bp)

Absent (108 bp)

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min.

RD12

GGGAGCCCAGCATTTACCTC GTGTTGCGGGAATTACTCGG AGCAGGAGCGGTTGGATATTC

Present (369 bp)

Absent (306 bp)

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min.