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Table 1 List of oligonucleotide sequences and cycling conditions used in the study

From: Prevalence and molecular characterization of Mycobacterium tuberculosis complex in cattle and humans, Maiduguri, Borno state, Nigeria: a cross-sectional study

Primer name

Primer sequence

(oligonucleotide sequences)

Product size

Enzyme activation

Cycles [45]

Incubation

Denaturation

Annealing

Extension

MYCGEN-F

MYCGEN-R

AGAGGTTGATCCTGGCTCAG

TGCACACAGGCCACAAGGGA

1030 bp

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min.

MYCGEN-F

MYCAV-R

AGAGGTTGATCCTGGCTCAG

ACCAGAAGACATGCGTCTTG

180 bp

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min

MYCINT-F

MYCGEN-R

CCTTTAGGCGCATGTCTTTA

TGCACACAGGCCACAAGGGA

850 bp

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min.

TB1-F

TB1-R

GAACAATCCGGAGTTGACAA

AGCACGCTGTCAATCATGTA

372 bp

95 °C

15 min.

94 °C

1 min

62 °C

1 min

72 °C

1 min

72 °C

10 min.