Primer name | Primer sequence (oligonucleotide sequences) | Product size | Enzyme activation | Cycles [45] | Incubation | ||
---|---|---|---|---|---|---|---|
Denaturation | Annealing | Extension | |||||
MYCGEN-F MYCGEN-R | AGAGGTTGATCCTGGCTCAG TGCACACAGGCCACAAGGGA | 1030 bp | 95 °C 15 min. | 94 °C 1 min | 62 °C 1 min | 72 °C 1 min | 72 °C 10 min. |
MYCGEN-F MYCAV-R | AGAGGTTGATCCTGGCTCAG ACCAGAAGACATGCGTCTTG | 180 bp | 95 °C 15 min. | 94 °C 1 min | 62 °C 1 min | 72 °C 1 min | 72 °C 10 min |
MYCINT-F MYCGEN-R | CCTTTAGGCGCATGTCTTTA TGCACACAGGCCACAAGGGA | 850 bp | 95 °C 15 min. | 94 °C 1 min | 62 °C 1 min | 72 °C 1 min | 72 °C 10 min. |
TB1-F TB1-R | GAACAATCCGGAGTTGACAA AGCACGCTGTCAATCATGTA | 372 bp | 95 °C 15 min. | 94 °C 1 min | 62 °C 1 min | 72 °C 1 min | 72 °C 10 min. |