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Correction: Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale
BMC Microbiology volume 22, Article number: 134 (2022)
Correction: BMC Microbiol 22, 103 (2022)
Nozaki H, Mori F, Tanaka Y, et al. Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale. BMC Microbiol. 2022;22:103. https://doi.org/10.1186/s12866-022-02519-9.
. List of strains of Gonium used in this study. Table S2. Specific primers used for genomic PCR for strains of Gonium pectorale (Additional file 1: Table S1). Table S3. Recovery results of vegetative cells of Gonium pectorale strain NIES-4502 after possible optimal cryogenic treatments (6% DMF; Table 1) in liquid nitrogen by using a simple cryopreservation module (Thermo Scientific™ Mr. Frosty™ Freezing Container, Thermo Fisher Scientific, Waltham, MA, USA) for two-step cooling in cryopreservation. Figure S1. Mating type determination of four newly established strains of Gonium pectorale (NIES-4499–4502, Additional file 1: Table S1) by genomic PCR of mating type minus-specific minus dominance gene (MID) and mating type plus-specific gamete plasma membrane protein gene (FUS1). Actin is an autosomal gene (control). For primers used, see Additional file 1: Table S2. A. NIES-4499. B. NIES-4500. C. NIES-4501. D. NIES-4502. For full-length original gel images, see Additional file 1: Figure S2. Figure S2. Full length, unprocessed gel images of the three genes shown in Additional file 1: Figure S1. Marker 6(λ/StyI digest)marker (NIPPON GENE, Tokyo, Japan) was used as a molecular size marker (1st, 6th, 11th and 17th lanes). 2nd, 3rd, 4th and 5th lanes: A, B, C and D, respectively, of MID (Additional file 1: Figure S1). 7th, 8th, 9th and 10th lanes: A, B, C and D, respectively, of Actin (Additional file 1: Figure S1). 12th, 13th, 14th and 15th lanes: A, B, C and D, respectively, of FUS1 (Additional file 1: Figure S1).
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Nozaki, H., Mori, F., Tanaka, Y. et al. Correction: Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale. BMC Microbiol 22, 134 (2022). https://doi.org/10.1186/s12866-022-02539-5