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Fig. 5 | BMC Microbiology

Fig. 5

From: Proteome and secretome profiling of zinc availability in Cryptococcus neoformans identifies Wos2 as a subtle influencer of fungal virulence determinants

Fig. 5

Assessment of thermotolerance, zinc utilization, and melanin production in C. neoformans. A Growth curve of WT, wos2Δ, and wos2Δ::WOS2 strains in zinc-replete (MM+ Zn 10 μM) at 30 °C and 37 °C. Growth curves measured at OD600nm. B Plate dilution assay of WT, wos2Δ, and wos2Δ::WOS2 strains in zinc-limited and zinc-replete media at 30 °C and 37 °C. Plate assays imaged after 3 d of growth. *Denotes significant difference between comparisons to WT by Student’s t-test, asterisks as follows: ***, P < 0.005. C L-DOPA plates for melanin assay at 30 °C and 37 °C for WT, wos2Δ, and wos2Δ::WOS2 strains. Plates imaged after 3 d. Experiment performed in biological triplicate and technical duplicate

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BMC Microbiology

ISSN: 1471-2180

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