Fig. 7

Streptomyces sp. TPP199 root colonization patterns targeted by 23S rRNA with Cy5 dye double-labeled HGC69a. Probe visualized by two-photon laser scanning microscopy where autofluorescence of the plant tissue is shown in blue (B, F, L, N, R) and green (C, G, K, O, S) separately, while the probe is in red separately (D, H, J, P, T) and in a merged image (A, E, I, M, Q). A-D Apical root from a not-inoculated plant, scale bar of 50 μm (day 3 of the assay). E-H Longitudinal histological section root from a not-inoculated plant, scale bar of 90 μm (day 10 of the assay). I-L Rhizodermis from a 3 day post-inoculated plant, scale bar of 20 μm. M-P Root hair growth area from a 3 day post-inoculated plant, scale bar of 60 μm. Q-T Longitudinal histological section root from a 10 day post-inoculated plant, scale bar of 60 μm. A, E, I, M, and Q merged image. B, F, J, N, and R blue (excitation-emission: 405/410–480 nm). C, G, K, O, and S green (excitation-emission: 488/490–560 nm) separately, while the 23S rRNA with Cy5 dye double-labeled HGC69a probe is in D, H, L, P, and T red separately (excitation-emission: 633/638–747 nm). Rz: rhizodermis, Pr: root hair. Mx: metaxylem. Yellow arrows indicate Streptomyces sp. TP199 targeted with Cy5 dye double-labeled HGC69a probe fluorescence, and white arrows indicate the plant morphology