Fig. 2
Circular dichroism analysis of KfrA proteins of R751 and RA3 plasmids. KfrA proteins were overproduced and purified by affinity and size-exclusion chromatography. a Molar ellipticity was measured at 0.8 μM KfrAR751 and 1.4 μM KfrARA3 concentrations at 200–260 nm wavelength range at three temperatures. b Melting curves for both KfrAs. The same concentrations of proteins were used as above. The CD signal was measured at 222 nM in the range of temperature 10 - 90 °C with an interval of 2 °C. Inlets correspond to Tm (melting temperature) calculations