Fig. 4

Mycophagous behaviour and secretion profile of T3SS signal deleted variant of Bg_9562 in NGJ1 strains. a Mycophagous activity of NGJ1 and its variant strains on R. solani on PDA plates. The Bg_9562 mutant (NGJ101) and T3SS signal deleted variant Bg_9562 expressing strain (NGJ104) were mycophagy deficient while the wild type NGJ1 showed enhanced mycophagy in presence of EGTA. b Western blot analysis (using Bg_9562 specific polyclonal antibody) of proteins isolated from the culture supernatant and bacterial pellets of NGJ1 and NGJ104 grown in PDB broth with EGTA/CaCl₂ supplementation. Similar results were obtained in at least three independent biological experiments and only representative photographs are shown