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Fig. 2 | BMC Microbiology

Fig. 2

From: Development of flow cytometry based adherence assay for Neisseria gonorrhoeae using 5′-carboxyfluorosceinsuccidyl ester

Fig. 2

Flow cytometric gating strategy on ME-180 cells. Gating strategy on live ME-180 cells forward/side scatter plot (a), ME-180 cells forward scatter/FL1 channel plot (b), ME-180 cells infected with non-CFSE stained N. gonorrhoeae (MOI 1:100) (c), ME-180 cells infected with CFSE-stained N. gonorrhoeae (MOI 1:100) without washings with PBS (d), ME-180 cells infected with CFSE-stained N. gonorrhoeae (MOI 1:100) with washings (n = 3) with PBS to remove loosely adhered bacteria (e), and ME-180 cells incubated in CFSE-bacterial supernatant forward scatter/FL1 channel plot (f)

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BMC Microbiology

ISSN: 1471-2180

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