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Table 2 Estimated mean MAP load determined by qPCR at different dilutions (10−2, 10−4 and 10−6) before (PRE) and after (POST) treatment with copper ions, for each replicate experiment. Data represent mean number of MAP DNA copies (genome equivalents) per 200 μL of PBS, from which DNA was extracted

From: In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions

Replicate experiment MAP dilution:
10−2 10−4 10−6
PRE POST PRE POST PRE POST
I 1.9 × 106 1.4 × 103 1.5 × 103 3.2 × 102 1.3 × 102 NDa
II 2.8 × 106 ND 5.5 × 102 ND 4.7 × 101 ND
III 9.4 × 105 1.2 × 101 9.2 × 102 1.6 × 101 3.3 × 102 ND
IV 1.9 × 105 1.1 × 101 1.5 × 103 1.2 × 101 2.7 × 102 ND
V 1.6 × 105 3.2 × 102 3.3 × 103 2.1 × 101 3.1 × 103 ND
  1. aND, no MAP DNA detected by qPCR