Fig. 6

Generation and immunoblotting of Hpa1 mutant versions compared with the canonical form. a Schematic diagrams showing full-length sequence and mutant versions of the Hpa1 protein. Mutants ∆N36, ∆Nα, ∆Cα, and αNC were generated by deleting the N-terminal region made of 36 residues, the N-terminal helix (Nα), the C-terminal helix (Cα), and both helices (NCα), respectively. Amino acid sequence borders of these protein variants are pointed by arrowheads linked to the numbers of initial and terminal residue sites. b Immunoblotting. Each of Hpa1 variants was fused to a GST tag. Fusion proteins were produced by prokaryotic expression and analyzed by Western blotting hybridization with the GST antibody