Fig. 5
Visualization of development of A. fumigatus conidia and hyphae in fungal nodules/granulomas in G. mellonella larvae inoculated with 1 × 106 viable conidia. Fungal nodules were dissected from larvae and stained with Calcofluor white and FM 4–64. Bright field images reveal the formation of melanized plaques (grey) and cellular infiltrates (green arrow). Fluorescent microscopy using Calcofluor white fluorescence revealed conidia germinating conidia (oval shaped; white arrow) at 6 h and dense hyphal infiltration (red arrow) at 24 h post infection. Corresponding images obtained from fungal nodules stained with FM 4–64 demonstrates the accumulation of hemocyte membrane at 6 and 24 h (scale bar corresponds to 20 μm)