Table 3 Primers and sequences used in this study for q-PCR
Name | Gene product | Primer | Sequence (5′➔3′) | qRT-PCR | Illumina | ||
|---|---|---|---|---|---|---|---|
Log2FC | Regulated | Log2FC | Regulated | ||||
U876_04005 | RTX toxin | F | GCCAAGAACCTGACCTAC | 0.78 | Up | 1.06 | Up |
| Â | R | TAACTACCGTCCGACCAT | Â | Â | Â | Â | |
U876_15265 | hemolysin | F | TGCTCGTACTTGCTGTTG | 3.85 | Up | 3.80 | Up |
| Â | R | GACTACCTGCTGCTGGAT | Â | Â | Â | Â | |
U876_15575 | secretin | F | CGATGCGTACCGATATGT | 5.00 | Up | 5.33 | Up |
| Â | R | AGACTAACAACCAGGATGAG | Â | Â | Â | Â | |
U876_16325 | type I secretion system | F | GCTCATCGCCTCAATACC | 1.42 | Up | 1.02 | Up |
| Â | permease/ATPase | R | TAGCCAGTGTGAGTCAGG | Â | Â | Â | Â |
U876_02495 | phosphatidylcholine-sterol | F | TTCGGTGTTCCAGCCATA | 2.34 | Up | 1.87 | Up |
| Â | acyltransferase | R | CCAAGTATCAGGTCATCAAC | Â | Â | Â | Â |
U876_00180 | lysophospholipase L2 | F | AGCACATAATCGTCAAACTG | 1.23 | Up | 1.51 | Up |
| Â | R | GCCATCCTCATCGTCAAC | Â | Â | Â | Â | |
U876_12850 | FAD/NAD(P)-binding | F | CGATTACCACAAGATTGACC | 2.34 | Down | −5.25 | Down |
| Â | oxidoreductase | R | TGATCCAGCAGCACTATG | Â | Â | Â | Â |
U876_06295 | HPr family phosphocarrier | F | CGGAGACCACAGTGATCT | −0.86 | Down | −2.07 | Down |
| Â | protein | R | TGTACGAGAAGTCTGTTGTT | Â | Â | Â | Â |
U876_06300 | cysteine synthase A | F | CAGAGCAATACCCGTGTT | 1.69 | Up | 1.99 | Up |
| Â | R | TCAACCGTGTTACCAAGG | Â | Â | Â | Â | |
U876_14760 | peptidase T | F | CCGAGGATCAAACCCATTC | −1.23 | Down | −6.23 | Down |
| Â | R | CTTGCCGTGGAAGTTGTG | Â | Â | Â | Â | |
U876_07265 | flagellar hook capping protein | F | CAATGTCGGTTACCTGGAA | −0.86 | Down | −1.05 | Down |
| Â | R | GTCCTTGTCCTTGCCATC | Â | Â | Â | Â | |
U876_07270 | flagellar hook protein FlgE | F | TCAGCGACCTACAGCAAT | 0.25 | Up | −1.25 | Down |
| Â | R | CACCAGACAGCAGAGACT | Â | Â | Â | Â | |
U876_12225 | murein transglycosylase A | F | CCAGACTGATGCCGTAAC | 1.25 | Up | −1.16 | Down |
| Â | R | CAAGATGACTCGTCGCTAC | Â | Â | Â | Â | |
U876_03850 | PAP2 family protein | F | GATGGTGCCGTTGTTCTC | 2.54 | Up | 2.07 | Up |
| Â | R | ACAGCAGTGGTAGACAGAG | Â | Â | Â | Â | |
U876_17510 | outer membrane protein | F | GGTGAGTGGAACGGTTAC | −0.99 | Down | −2.14 | Down |
| Â | R | ATCGGAGTGCCAGTAGATA | Â | Â | Â | Â | |
U876_18585 | hemin ABC transporter | F | CGATCTGGTGCTGGTTAG | 4.71 | Up | 7.32 | Up |
| Â | substrate-binding protein | R | CTTGATCCACTTGGCGAT | Â | Â | Â | Â |
U876_21455 | TonB-dependent siderophore | F | CGTCTCAGTCACCAGTCT | 2.61 | Up | 6.07 | Up |
| Â | receptor | R | ATCCAGGTTGTTGTTCTTGT | Â | Â | Â | Â |
U876_20975 | transcriptional activator | F | TTGAACAGCACCACCTTG | 3.33 | Up | 1.22 | Up |
| Â | protein AhyR/AsaR | R | GCTTGAGTACCTCGAACAT | Â | Â | Â | Â |
U876_23540 | LuxR family transcriptional | F | GAAGGAGTGCCTGTTCTG | 1.14 | Up | 1.45 | Up |
| Â | regulator | R | TATGATGCCGCTGGAGAT | Â | Â | Â | Â |
U876_09860 | 2,3-dihydroxybenzoate-AMP | F | TACAGGATGCCGATGGTTA | 6.20 | Up | 9.23 | Up |
| Â | ligase | R | ATCCGTGCTGACGATGAA | Â | Â | Â | Â |
U876_01300 | DNA-directed RNA | F | GGATCACGGTGCCTACAT | Â | Â | Â | Â |
(rpoB) | polymerase subunit beta | R | TAACGCTCGGAAGAGAAGA | Â | Â | Â | Â |