Table 2 Primers used in this study
Primer | Sequence (5′ to 3′, underlined sequences are designed restriction sites) | Description | Reference |
|---|---|---|---|
pr31 | GGTGAGCTCTATTCTTTATTGCCGATTATC | For icd insertional mutant, forward, SacI | This study |
pr32 | AAATCTAGAGTAAGTCGCTTTAATCGCTTC | For icd insertional mutant, reverse, XbaI | This study |
pr50 | AAAGAGCTCGTGATCCAGATGTCGATGCTA | For sucA insertional mutant, forward, SacI | This study |
pr51 | GGTTCTAGAGTTCAGTGTCGATAATGTGCA | For sucA insertional mutant, reverse, XbaI | This study |
pr52 | AAAGAGCTCGGTCGGATTAGTACAGCGAAG | For sucC insertional mutant, forward, SacI | This study |
pr53 | GGTTCTAGACTTTTTCAATTTCCACGCCGC | For sucC insertional mutant, reverse, XbaI | This study |
pr54 | AAAGAGCTCATGTTCGTTGCGGTCGGAATT | For sdhC insertional mutant, forward, SacI | This study |
pr55 | GGTTCTAGATCCAACTCTTCAAAGTGGCCC | For sdhC insertional mutant, reverse, XbaI | This study |
pr56 | GGTGAGCTCTCCTTGCACCATATTGATATG | For fumA insertional mutant, forward, SacI | This study |
pr57 | GGGTCTAGAAGGCTTATCATCGAGAAGAGAG | For fumA insertional mutant, reverse, XbaI | This study |
pr29 | GGTGAGCTCATGCCAGCGTTAACATTAAAC | For mdh insertional mutant, forward, SacI | This study |
pr30 | AAATCTAGAGCTGTATGACATCGCACCGGT | For mdh insertional mutant, reverse, XbaI | This study |
pr33 | AAAGAGCTCGCGGCGTGAGACTAAGGCATC | For cra insertional mutant, forward, SacI | This study |
pr34 | AAATCTAGACAATGGCAAAGCGCAGAAGTA | For cra insertional mutant, reverse, XbaI | This study |
vah1 F RT | GTTTGGTATGGAACACCGCTCAAG | For vah1 qRT-PCR, forward | This study |
vah1 R RT | GGCTCAACCTCTCCTTGTAACCAA | For vah1 qRT-PCR, reverse | This study |
plp F RT | CAGACGACCACCAGTAACCACTAA | For plp qRT-PCR, forward | [8] |
plp R RT | GCAATCATGATGACCCAGCAACAG | For plp qRT-PCR, reverse | [8] |
Pm111 | GGAAATTATTCCGCCGACGATGGA | For rtxA qRT-PCR, forward | [7] |
Pm112 | GCCGATACCGTATCGTTACCTGAA | For rtxA qRT-PCR, reverse | [7] |