Fig. 6From: A rapid seamless method for gene knockout in Pseudomonas aeruginosa Screen of deletion mutants by colony PCR. Lane 1, 400 ng 1 kb DNA ladder; lane 2–15, 10 μl of PCR products of 14 colonies randomly selected from the TYS10 plate. Of the fourteen randomly selected colonies, 8 colonies were deletion mutants while six colonies reverted to wild type during the second recombination eventBack to article page