Open Access

Erratum to: Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage

BMC MicrobiologyBMC series – open, inclusive and trusted201717:180

https://doi.org/10.1186/s12866-017-1072-9

Published: 21 August 2017

The original article was published in BMC Microbiology 2011 11:211

Erratum

After publication of our article [1], we became aware that during the assembly of panel A in Fig. 6 a wrong control lane (α T7) had been included. The results and conclusions are not affected by this error.

The corrected Fig. 6 is shown as follows:

Fig. 6

Presentation of the antigenic tags on gp9 of phage particles. (a) M13 phage (panel a) was applied onto nitrocellulose membrane and incubated with antibody to gp8, T7tag and HA tag, respectively, at the indicated concentrations. To visualize the phage a peroxidase-linked secondary antibody was applied and analysed by chemoluminescence. b M13 am9 phage grown in E. coli K38 cells bearing a plasmid encoding gp9-T7 or gp9DT7, respectively, were incubated with antibody to T7 and treated as described above. c M13 am9 phage propagated in E. coli K38 cells bearing a plasmid encoding gp9-HA or gp9DHA, respectively, were incubated with antibody to HA and treated as described above. For controls (Ctr), uninfected cultures were tested under identical conditions.

Notes

Declarations

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Institute of Microbiology and Molecular Biology

Reference

  1. Ploss M, Kuhn A. Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage. BMC Microbiol. 2011;11:211.View ArticlePubMedPubMed CentralGoogle Scholar

Copyright

© The Author(s). 2017

Advertisement