Skip to main content

Table 1 Characterization of mAbs reactive to the epitopes within Omp31 of B. melitensis

From: Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies

MAb Isotype Peptide ELISA rOMP31 Western-Blot NMP Western-Blot NMP
ELISA
SSP
ELISA
TC
IFS
B. m
ICS
Epitope (aa)/peptide
1H2 IgG1 (K) 12 ++ <1 1 + L (33–48/42–57)/P05/06
2D2 IgM (K) 7 ++ ++ 4.5 4.8 + ++ L (33–48/42–57)/P05/06
2G9 IgG1 (K) 3 ++ ++ <1 2.2 + + L (33–48/42–57)/P05/06
7A3 IgG1 (K) 1.2 ++ ++ 7 17 + + L (33–48/42–57)/P05/06
5B1 IgG2a (K) 3.8 + 1.2 2 + ++ L (87–102)/P11
2C1 IgG2a (K) 2.7 ++ ++ 2.4 5.5 ++ ++ L (159–174/168–183)/P19/20
2E7 IgG2a (K) 1.8 ++ ++ 1.5 3.5 ++ ± L (159–174/168–183)/P19/20
4E9 IgG2a (K) 3.7 + ++ 2 5 ++ ++ L (159–174/168–183)/P19/20
4H10 IgG2a (K) 1.9 ++ ++ 2.2 4.2 ++ L (159–174/168–183)/P19/20
8F11 IgG2a (K) 2.2 ++ ++ 2.5 5.5 ++ + L (159–174/168–183)/P19/20
2A8 IgG1 (K) 7.8 ++ + <1 1.5 (+) + L (177–192)/P21
6D8 IgG1 (K) 4.7 ++ <1 <1 + ± L (177–192)/P21
5B3 IgG2a (K) 2.8 ++ + <1 16 + ++ L (204–219)/P24
2B6 IgM (K) <1 ++ 8 11 + SC(20–240)
4H6 IgM (K) <1 ++ <1 1.2 ± SC(20–240)
5B11 IgM (K) <1 ++ 5.8 9.2 ± SC(20–240)
5H3 IgG2a (K) <1 ++ ++ 11 18 + ++ SC(20–240)
6E10 IgG2a (K) <1 + + 1 2 ++ SC(20–240)
6F9 IgG2a (K) <1 + ++ <1 1.5 ++ ± SC(20–240)
11C4 IgG2a (K) <1 ++ ++ 1.2 2.6 ++ ± SC(20–240)
2H5 IgM (K) <1 4.1 7 C (20–240)
5F11 IgM (K) <1 8.2 12 + C (20–240)
  1. NMP, native membrane proteins extracts of B. melitensis; SSP, supernatant of sonicated B. melitensis proteins; TC IFS, Lentivirus-mediated Omp31, transduced 293Tcells detected by immunofluorescent staining; B.m ICS, B. melitensis strain detected by immunochemical staining; L, linear; SC, semi-conformational; C, conformational. The numbers indicate the S/CO values in the EIA; the reactivity levels are indicated by ++ (strongly reactive), + (reactive), ± (indeterminate) or – (non-reactive) in the Western-blot, IFS and ICS. (+), indicate false positive for the negative control of cells. Five IgG and three IgM clones in bold are representatives of high capacity of mAbs reacting with native Omp31 antigens of B. melitensis in various assays.