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Fig. 6 | BMC Microbiology

Fig. 6

From: In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp.

Fig. 6

RT-qPCR quantitation of trpBA gene mRNA, isolated from HEp-2 cells, infected with C. trachomatis strain D during tryptophan starvation conditions and rescue. C. trachomatis infected cultures and conditions were as described in the legend of Figs. 4 and 5. a Total RNA was isolated from infected cultures grown in tryptophan-depleted media at 72 h PI (‘No rescue’), and after indole rescue in different concentrations (0.5, 5 μM), as well as complete DMEM conditions harvested at 36 and 72 h PI (‘DMEM’). No rescue treatment is significantly different p < 0.0001. b trpBA transcript levels were measured from rescue treatments of infected cultures using indole positive bacterial supernatant (P. intermedia, P. nigrescens) and indole negative (P. bivia), applied in dilution of 1:10,000. P. bivia is significantly different p < 0.005

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