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Fig. 5 | BMC Microbiology

Fig. 5

From: In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp.

Fig. 5

Recovery of tryptophan-starved C. trachomatis strain D after rescue with secretions from five (Chlamydia positive/negative) participants that have different concentrations of indole in their vaginal secretions. Monolayers of HEp-2 cells were seeded in the presence of tryptophan-depleted media 24 h before infection. Cells were infected with C. trachomatis D, at an MOI of 0.5, and were incubated for 36 h. The Chlamydia infected cultures were allowed to recover for 36 h in the presence of secretions from two C. trachomatis negative participants (111 and 112) and three C. trachomatis positive participants (213, 211 and 306). Chlamydia test (CT+/CT-), pH and BV conditions are indicated in boxes above each participant. Secretions were added to the cultures at dilution of 1:100. Axis X represent the indole concentrations measured from the participants’ secretions, axis Y represent the recovery effect of the Chlamydia in IFU/ml. Infected cells and culture supernatants were sonicated and used to infect a new HEp-2 cell monolayer for enumeration of recoverable IFUs. Data are presented as the mean ± SD IFU/ml (n = 9) determinations

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