Table 2 Primers used in the study
Gene Name | Experiment | Primer | Sequence (5′ to 3′) |
|---|---|---|---|
MAV_0502 | BlaC | F | GGATCCATGCTCGACGCCGTG |
R | GAATTCTTGCCCCGCAATGAGAACG | ||
RT-PCR | F | GTGAGCTGCAATGTCGGATG | |
R | GCAGTACTGGTGCAGATCGT | ||
MAV_0516 | BlaC | F | GGATCCATGCCACAGGGAACTGTG |
R | GAATTCCAGGGAGCGGACTCCG | ||
RT-PCR | F | ACAGGGAACTGTGAAGTGGTT | |
R | GATTTCGAACTCGACCTTCTGG | ||
MAV_0628 | BlaC | F | GGATCCATGAGCACATCCAACACAGTC |
R | GAATTCGGGCGGGCAGGAGTG | ||
RT-PCR | F | TCGCCGAGGGGTTACTCA | |
R | AGGTAGTCGATGACGTTGCC | ||
MAV_1356 | BlaC | F | GGATCCATGGCGGCGATGAAG |
R | GAATTCGCTGGAGCTCGTGACG | ||
RT-PCR | F | ATGGCGGCGATGAAGCC | |
R | CTTGAGTTCGTCACGGAGGG | ||
MAV_4394 | BlaC | F | GGATCCGTGAAAACTCACCGGATCG |
R | GAATTCGACGAGAGGTGCTTGCGAAG | ||
RT-PCR | F | CCTACGGGGTCAACTATGCC | |
R | CACGATGTCCATCACCGAGG | ||
MAV_1419 | BlaC | F | GGATCCAATGTTCTCGCGCCGCATCATCACC |
R | GAATTCGGCGGGCTGGCCGGAGAATTGCGGG | ||
RT-PCR | F | CGATGAGATGTTCCTCGCCC | |
R |
| ||
MAV_0398 | BlaC | F | GGATCCAATGACCATGCGGATCACGCGGGTTTGCCGGGCGGT |
R | GAATTCTTGACCCAGCGCGTTTTGCATGGCCCCGG | ||
RT-PCR | F | GACGAGAACTGGACCAAGCA | |
R | TATTGACCCAGCGCGTTTTG | ||
MAV_1177 | BlaC | F | GGATCCATGAGCATCAACTACCAGTTC |
R | GAATTCGGCCCAGCTGGACC | ||
RT-PCR | F | ACTACCAGTTCGGCGATGTC | |
R | CGACCCAACTGGGTGATGAA | ||
MAV_1178 | BlaC | F | GGATCCATGGCAACACGTTTTATGACTG |
R | GAATTCGCTGCTGAGGATCTGCTG | ||
RT-PCR | F | TTATGACTGACCCGCACGAA | |
R | GTTGACGATGTTGCGGAAGG | ||
MAV_4077 | BlaC | F | GGATCCAATGGGGGACCAGCAGAGCGGGCCGCAGGAA |
R | GAATTCTTGGTTCTTCTTCTGGCGCT | ||
RT-PCR | F | GAAGGGCAAGGCCAAGGA | |
R | TCTTCTTCTGGCGCTCCTC |
