Fig. 3

Survival rate of M. alhagi after H₂O₂ treatment. Wild-type (WT), ΔprkA (prkA deletion mutant), and CΔprkA (complement of ΔprkA) bacterial cells were grown to OD₆₀₀ ≈ 0.8 in TY broth medium, adjusted to OD₆₀₀ ≈ 0.1, and then treated with 10 mM H₂O₂ for 30 min or incubated without H₂O₂ in otherwise identical conditions as a control. The percentage survival rate of the three stains was calculated as follows: [(CFU per ml after treatment with H₂O₂)/(CFU per ml before treatment with H₂O₂)] × 100. Data shown are the means of three independent experiments and the error bars indicate standard deviations. The significance of differences is shown at the P < 0.05 level (t-test). Different lowercase letters mean significant difference between two columns