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Fig. 1 | BMC Microbiology

Fig. 1

From: Functional analysis of PrkA - a putative serine protein kinase from Mesorhizobium alhagi CCNWXJ12-2 - in stress resistance

Fig. 1

Sensitivity of the wild-type and mutant strains to NaCl and alkaline. Wild-type (WT), ΔprkA (prkA deletion mutant), and CΔprkA (complement of ΔprkA) bacterial cells were grown to OD600 ≈ 0.8 in TY broth medium, adjusted to OD600 ≈ 0.2, and then serially diluted and spotted onto TY agar plates containing no additional NaCl or 0.4 M NaCl at neutral pH, and TY agar plates at alkaline pH (pH 9). Ten-fold serial dilutions are shown. The plates with no additional NaCl were incubated at 28 °C for 3 days, while the plates with 0.4 M NaCl and the plates at pH 9 were incubated for 5 days at the same temperature

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