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Fig. 8 | BMC Microbiology

Fig. 8

From: The MarR-like protein PchR (YvmB) regulates expression of genes involved in pulcherriminic acid biosynthesis and in the initiation of sporulation in Bacillus subtilis

Fig. 8

YvmB binding to the yvmB-yvmC intergenic region and to the yvnB and yisI promoter regions. a Location of the three identified YvmB boxes in the promoter region of yvmB. The −10 and −35 regions similar to the consensus sequence of σA-dependent promoters are indicated: white boxes. Location of the palindromic sequence, palindrome I, is indicated in blue. Location of the palindromic sequence, palindrome II, is indicated in green. Location of the three YvmB boxes is indicated in red. The DNA fragments P1, P2 and P3 used to perform gel shift assays are diagrammed. The DNA probes used were: (b) probe P1, probe P2 and probe P3; (c) probe P-yvnB and (d) prove P-yisI. Increasing concentrations of purified YvmBHis-tag were used (nM)

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