Table 1 Bacterial strains, plasmids and oligonucleotides used in this study
From: Plasmid pPCP1-derived sRNA HmsA promotes biofilm formation of Yersinia pestis
Strains | ||
|---|---|---|
Name | Description | Sources |
WT | Y. pestis wild-type strain 201 | [36] |
WT::pBAD | Y. pestis WT strain carrying plasmid pBAD-TF | [39] |
∆pPCP1 | Y. pestis WT strain lacking plasmid pPCP1 | [38] |
∆hmsA | hmsA deletion mutant derived from Y. pestis WT strain 201 | [28] |
∆hmsA::pBAD | ∆hmsA strain carrying plasmid pBAD-TF | This study |
∆hmsA::HmsA | ∆hmsA strain carrying plasmid pBAD-HmsA | This study |
∆hmsS | hmsS deletion mutant derived from Y. pestis WT strain | [18] |
∆fur | fur deletion mutant derived from Y. pestis WT strain | [18] |
∆hmsB | hmsB deletion mutant derived from Y. pestis WT strain | [35] |
Plasmids | ||
pRW50 | A low-copy lacZ fusion vector | [42] |
pBAD-TF | Transcriptional fusion vector modified from pBAD/HisA | [39] |
pBAD-HmsA | HmsA overexpressing plasmid by inserting a DNA fragment amplified by primer HmsA-pBAD-F/R into pBAD-TF | This study |
Oligonucleotides | ||
Name | Sequence (5′-3′) | |
HmsA-pBAD-F/R | CACGAATTCGCAAAAGTCAGGACTAGACATATTAAC/TTGAAGCTTACCACATAAAAAAGGCCCTCACAGG | |
HmsA-NB-F/R | CACGAATTCGCAAAAGTCAGGAC/AATTGTAATACGACTCACTATAGGGCGTTGAAGCTTACCACATAAAAAAGGC | |
HmsB-NB-F/R | TGTAATAATAGTCATATCATCGTAAATTTGAATTGTAATACGACTCACTATAGGGCGTGCTTCAGTGGCTCATGTC | |
HmsA-PE-R | TCTGTGAGGGCGTATGATAAGG | |