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Fig. 7 | BMC Microbiology

Fig. 7

From: Plasmid pPCP1-derived sRNA HmsA promotes biofilm formation of Yersinia pestis

Fig. 7

Regulatory effects of HmsA on rovA. a Primer extension results. The relative levels of hmsH transcript were determined in the WT and ∆hmsA mutant strains by primer extension assays. The Sanger sequence ladders (lanes G, C, A and T) and the primer extension products of hmsH were analyzed on an 8 M urea-6 % acrylamide sequencing gel. The transcription start sites of hmsH were indicated by arrows, and the minus number under the arrow indicates the nucleotide position upstream of the hmsH start codon. b qRT-PCR results. The relative levels of the hmsH transcript were determined in the WT and ∆hmsA mutant strains by qRT-PCR. c lacZ fusion results. The hmsH::lacZ transcriptional fusion vector was transformed into the WT and ∆hmsA mutant strains. The transcriptional activity of hmsH determined in the bacterial cellular extracts was represented as Miller units of β-galactosidase activity

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