Fig. 6
From: Plasmid pPCP1-derived sRNA HmsA promotes biofilm formation of Yersinia pestis
Regulatory effects of HmsA on HmsB. a Primer extension results. b Confirmation of HmsA and HmsB by northern blot analysis. The transcript of hmsB/hmsA in the WT and ∆hmsA/∆hmsB mutant strains was monitored by northern blotting (upper and bottom panel). c Measurement of the half-life of HmsB. The WT and ∆hmsA strains were grown to exponential phase at 26 °C and then treated with 250 μg/mL of rifampicin. Culture samples were collected at 0, 2, 4, 8, 16 and 32 min and were subject to RNA extraction and northern blotting using 5S rRNA and HmsB probes