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Fig. 1 | BMC Microbiology

Fig. 1

From: Plasmid pPCP1-derived sRNA HmsA promotes biofilm formation of Yersinia pestis

Fig. 1

Biofilm formation capacity and c-di-GMP production mediated by HmsA. a Bacterial colony morphology. The WT::pBAD, ∆hmsA::pBAD and ∆hmsA::HmsA strains were incubated on LB agar with 0.1 % arabinose designated as “+Ara” (middle row). The ∆hmsS or ∆fur strains were used as negative or positive controls for this assay, respectively (bottom row). b Crystal violet staining. Biofilms were quantified by crystal violet staining in the WT, ∆hmsA and ∆hmsA::HmsA strains grown in LB medium with the addition of 0.1 % arabinose. The ∆hmsS strain was grown under the same conditions as the negative control. Data are presented as the average of three separate experiments and error bars represent the standard deviation. c C. elegans biofilms. The percentage of L4/adults after incubation of nematode eggs on a lawn of the indicated Y. pestis strains was used to evaluate the capacity for biofilm formation. d Intracellular c-di-GMP concentration

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