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Table 1 Bacterial strains used in this study

From: ClpP-deletion impairs the virulence of Legionella pneumophila and the optimal translocation of effector proteins

Strain

Characteristics

Reference or source

E.coli DH5α

host strain used for cloning

Lab collection

Lp02

Virulent L. pneumophila serogroup 1, strain Philadelphia, rpsL, HsdR − , Thy −

Lab collection

Lp03

Virulent L. pneumophila serogroup 1, strain Philadelphia, dot03, rpsL, HsdR − , Thy −

[58]

Xp02

Lp02 with clpP deletion

This study

Xp02c

Xp02 containing pXL901 for complementation

This study

Lp02pj

Lp02 containing pJB908

This study

Lp03pj

Lp03 containing pJB908

This study

Xp02pj

Xp02 containing pJB908

This study

Lp02pGX*

Lp02 containing plasmids pGB908X*

with icm promoter-gfp fusions

This study

Xp02pGX*

Xp02 containing plasmids pGB908X*

with icm promoter-gfp fusions

This study

Lp03pG9

Lp03 containing plasmid pGB9089

with icmV promoter-gfp fusion

This study

  1. The X* represents the numbers of the plasmids or bacterial strains containing the icm promoter-gfp fusions: 1. icmTS, 2. icmR, 3. icmQ, 4. icmPO, 5. icmMLKEGCD, 6. icmJB, 7. icmHF, 8. icmWX, 9. icmV