Fig. 4

Analysis of the promoter activitiy and the expression of the dot/icm components. a-c Promoter activities of 9 dot/icm operons were examined through streaking and comparing the bacterial strains harboring the promoter-gfp fusion plasmids on plates. d Quantified fluorescence intensities of the wild-type Lp02 and the clpP mutant Xp02 harboring icmR:gfp fusion plasmid. There is no statistical difference between the two samples. e Western blot analysis of the expression of DotH, DotI, DotG, IcmS and IcmW in the wild type and the clpP deficient mutant strain. A western blot using antibody to isocitrate dehydrogenase (ICDH) was used to confirm even protein levels of the samples. Shown are the averages and standard deviations of 2 to 3 independent experiments, each performed in triplicate