The bactericidal effect of an ionizer under low concentration of ozone
© The Author(s). 2016
Received: 3 November 2015
Accepted: 22 July 2016
Published: 30 July 2016
Several mechanisms have been suggested for the bactericidal action of ionizers including electrical phenomena, effects of negative and positive ions and electrostatic repulsion. Negative and positive ions have indeed been shown to have bactericidal effects. In addition, since ozone is generated along with ions, these may contribute to the bacterial killing. In this study, we used a newly developed ionizer, which generates a relatively low concentration of ozone, to determine whether its effect on bacterial cells were due to ions or ozone, and, if ions, how the ions exerted their effects.
The effect of ions on bacterial killing was compared with that of the ozone produced using an ion trap to remove the ions. The ionizer had the ability to kill the bacteria, and ion capture dramatically reduced its bactericidal effect, indicating that the ozone generated had little or no bactericidal effect under these conditions, and the ions produced were responsible for almost all the bacterial killing. Operation of the ionizer increased the level of 8-oxo-dG, a marker of oxidative DNA damage, and decreased aconitase activity, which is known to be sensitive to ROS. The ionizer further affected the adenylate energy charge of bacterial cells. Removal of the ions with the ion trap greatly reduced all these effects.
These results indicate that negative and positive ions generated by the ionizer are responsible for inducing oxidative stress and so reducing bacterial survival.
Negative and positive ions have been shown to have bactericidal effects on various bacterial species and fungi [1–5]. Recently, air ionizers have been installed to prevent bacterial infections during operations  and infections of items of plastic medical equipment . Although, many studies indicate that negative and positive ions have bactericidal effects, the exact mechanisms involved remain unknown. Also some have argued that the bactericidal effects of ionizers are either overestimated  or due to ozone  or physical effects .
Oxidative stress is defined as a disturbance of the prooxidant/antioxidant balance in favor of prooxidants, leading to potential damage to the cell . Excess prooxidants result in oxidative stress, which damages cell components such as lipids, proteins and DNA by oxidation [10, 11]. Oxidative stress-induced free radicals are highly reactive elements such as reactive oxygen species (ROS), which can attack biological molecules and lead to death.
A DC voltage applied to an ion generator will create a corona discharge at the end of the electrodes forming both ions and ozone. The amounts of ozone generated vary with the generator, and its effects on bacteria also differ . In this study, we used an ion generator, which generates a relatively low concentration of ozone, with the aim of clarifying whether its effect on bacterial cells was due to ions or ozone, and, if ions, how ions exerted their effects.
Generating air ions
Bacterial strains and culture conditions
Escherichia coli ATCC 23736, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Enterococcus faecalis ATCC 19433 were used. Cultures were grown on LB at 37 °C in a shaking incubator at 200 rpm for 16 h.
Exposure to negative and positive ions
Ion exposure experiments were performed in a sealed plastic chamber (46*46*46 cm, 100 L) at room temperature and 60–70 % relative humidity (RH). Temperature and RH were monitored with Thermo Recoder TR-72Ui (T&D Co, Japan). Cultures were serially diluted with diluent (1:100 LB), and deposited by suction onto 0.45 μm membrane filters (Φ47mm, Porafil, Germany) with a vacuum pump (Gast, USA) for 10 s. They were placed in empty Petri-dishes without lids, and then exposed to the fan (12 V, 1.5 m/s, Cooler Master, Taipei) only (F mode), the fan plus ionizer (IZ + F mode), or the fan plus ionizer and ion capture (IZ + F + C mode) for 30 ~ 120 min. In the IZ + F + C mode, the input to the ionizer was adjusted to 14 V to achieve the same level of ozone production, while it was 12 V in IZ + F mode (discussed in Results). After exposure, the membranes were incubated on NA agar plates for 48 h at 37 °C.
The experiments were performed with two sets of filters; one containing ~103 cfu/filter for percent survivals less than 10 %, usually exposed for 60–120 min, and the other containing ~102 cfu/filter for percent survivals exceeding 10 %, usually exposed for 30–60 min. Control filters were placed on NA plate immediately after suction and incubated. Survival was measured by colony count. For experiments on sessile bacteria on agar plates, we used Φ60mm plates, on which the number of bacteria would not exceed 100 cfu/plate, to maintain RH in the chamber below 75 %. Concentrations of ions were measured with an Air Ion Counter (Model: COM-3400, COM Systems Inc., Japan). For ion measurements the ion monitor was installed at the bottom of the chamber at three points alongside the membrane filters.
Ozone concentrations were determined by both an ozone meter (OZ 2000G), and the indigo method. The ozone meter was used to determine the concentration of ozone in the chamber, and the indigo method to make sure that the ozone reached the same concentration in conditions with and without ion capture. In the case of the ozone meter, the air sampler was placed at the bottom of the chamber and the air was sampled every 15 min for 2 h. For the indigo method, 12.5 μM of potassium indigotrisulfonate (Sigma, Inc., St Louis, MO, USA) solution was exposed in a 60Φ dish with stirring. Decolorization of the blue potassium indigotrisulfonate solution by dissolved ozone was measured with a SpectraMax M2 multi-microplate reader (Molecular Devices) at 600 nm.
Ions were eliminated with an ion collector. Its principle is similar to that of electrostatic precipitation, which is a technique for removing particles suspended in air using an electrostatic force. The electric ion collector is composed of a low-voltage power supply and a collecting electrode. The input voltage of the power pack is 12 V in DC (Direct Current). Four wire-mesh layers are used for the collecting electrode; when positive and negative air ions pass through the wire mesh they are collected on the electrodes by applied negative and positive high voltages.
Intracellular reactive oxygen species (ROS) were measured using an intracellular ROS assay kit (Cell Biolabs, Inc., San Diego, CA, USA). Cultured bacteria were incubated with 100 μM 2′, 7′-dichlorodihydrofluorescine diacetate (DCFH-DA) for 1 h at 37 °C in the dark, to preload them with the DCHF-DA probe. They were then washed with PBS and resuspended in a 60Φ cell culture dish and exposed to F-mode, IZ + F mode or IZ + F + C mode treatment, with stirring for 120 min. After exposure, the cells were harvested and transferred to black cell culture fluorometric 96-well plates and their DCF fluorescence intensity was measured at excitation wavelength 480 nm, emission wavelength 530 nm and 530 nm cutoff. For DCF fluorescence images, bacterial suspensions were loaded on glass slides after incubation with DCFH-DA for 1 h, and exposed to F-mode, IZ + F mode and IZ + F + C mode, respectively, for 120mins. DCF fluorescence signals were detected with a confocal microscope (Olympus FV1200, Japan).
Measurement of 8-Oxo-dG
To examine if the ions generated caused oxidative damage to bacteria, we analyzed oxidative damage to DNA. A sample (0.1 ml) of an approximately 108 CFU/ml culture of S. aureus was filtered onto a 0.45 μm membrane filter, and exposed to the ionizer. After exposure, the bacteria were suspended in 5 ml of saline and sonicated briefly, and the suspension was centrifuged. DNA was extracted using a DNA extraction kit (iNtRON Biotechnology). The concentration of DNA was adjusted to 200 μg/ml by reading absorbance at 260 nm using a spectrophotometer (ND-1000, NanoDrop Technologies), and the formation of 8-oxodeoxyguanosine (8-Oxo-dG), a marker of oxidative DNA damage , was measured with an Oxiselect oxidative DNA damage ELISA kit (Cell Biolabs, Inc., San Diego, CA, USA).
Assay of aconitase activity
We also analyzed aconitase activity, which is reported to be sensitive to oxidative damage. 0.1 ml of an approximately 108 CFU/ml culture of S. aureus was filtered onto a 0.45 μm membrane filter, and exposed to the ionizer. After exposure, the bacteria were suspended in 5 ml of saline, resuspended by brief sonication and collected by centrifugation. They were then broken with a microbead beater, and enzyme activity was measured with an Aconitase Activity Colorimetric Assay Kit (Biovision) after adjusting concentrations of protein based upon absorbance at 562 nm (BCA Protein Assay Kit, PIERCE).
Adenylate energy charge (AEC)
Cultures were serially diluted and deposited on 0.45 μm membrane filters by suction, then exposed to the various treatment modes. Preheated boiling buffer solution (50 mM Tricine, 10 mM MgSO4, and 2 mM EDTA at pH 7.8) was added to the samples and the mixtures were heated for 3 min. The boiled extracts were chilled on ice for a minimum of 10 min and kept at room temperature. To measure ATP, extracts were added to reaction buffer (75 mM Tricine, pH7.5; 5 mM MgCl2, and 0.0125 mM KCl). To measure ADP + ATP, extracts were added to the same reaction buffer with addition of 0.5 mM phosphoenolpyruvate (Sigma) and 0.4 μg/μl of pyruvate kinase (Sigma). AMP + ADP + ATP was measured by further addition of 0.5 μg/μl of adenylate (myo) kinase (Sigma) to the buffer. The ATP and ADP + ATP mixtures were incubated for 30 min at 30 °C, and the AMP + ADP + ATP mixtures were incubated for 90 min at 30 °C, and all were placed in a boiling water bath for 3 min to stop the reactions, chilled on ice and kept at room temperature. ATP was then determined with luciferin/luciferase using Luciferase Assay Reagent (Promega) and a SpectraMax L Luminometer (Molecular Devices). AEC was calculated as described by Atkinson [(ATP) + 0.5 (ADP)] / [(ATP) + (ADP) + (AMP)].
Survival of bacteria after ionizer exposure
Since we had shown above that the ionizer that we used generated only small amounts of ozone making an insignificant contribution to the bactericidal effect, we attempted to determine the basis of the bactericidal effect of the ions. In the following experiments, we used S. aureus only, as it was relatively stable under our control conditions (F- mode).
Oxidative DNA damage
Since ROS were detected in S. aureus exposed to the ionizer, we examined the well-known targets of ROS. When an S. aureus suspension was exposed to ions generated by the ionizer, the level of 8-oxo-dG, an oxidized derivative of deoxyguanosine, increased dramatically with time (Fig. 5b). By contrast, air flow by fan (F-mode) or the ion-capture mode (IZ + F + C mode) did not elevate 8-oxo-dG.
Enzymes containing catalytic [4Fe-4S] clusters, such as aconitase, are thought to be important targets of ROS . We prepared cell-free extracts of S. aureus exposed to the ionizer, and found that the activity of aconitase decreased by about two-thirds after ion exposure, as expected if ROS were being produced (Fig. 5c).
Energy charge values
Energy charge is considered a metabolic control parameter. Viability is maintained at values of adenylate energy charge between 0.8 and 0.5, and cells die at values below 0.5. [18–20] We measured the effect of the ionizer on adenylate energy charge. In fan only mode, the adenylate energy charge was about 0.85 (Fig. 5d). In IZ + F mode the energy charge fell to about 0.7.
There has been much interest in the use of air ionizers to control infections in the air or on surfaces, and there have been a number of studies of their killing effects on bacteria and fungi [1–5]. However, it has not been clear to what extent the effects of ionizers are due to the ions or to the ozone produced .
In our experiments we used a newly developed ionizer that produces relatively small amounts of ozone (Fig. 3). The plasmaster 2.5G that we used generated about 35 ppb of ozone over 2 h in 100 L; however, in previous reports using ionizers ozone concentrations were reported to be from 100 ppb (8) to 2.3 ppm (3). The concentrations of ozone and ions are affected by the volume of the chamber and the closeness of the generator and detector. In the conditions we used, the results showed that very little of the bactericidal effect of this ionizer was due to ozone because ion capture virtually abolished it. We were therefore able to analyze how ions, specifically, exert their effects on bacteria.
In previous studies most cell killing was examined on sessile cells on agar plates. With the ionizer used in this study, however, it was not easy to detect any effects in a 100 L chamber over two hours of exposure. When we used the plasmaster 2,5G on sessile cells, although Bacillus subtilis survival was reduced to less than 10 % after 2 h, the other 3 strains tested remained unaffected over 2 h (Additional file 1: Figure S1). We were able to detect some killing of the other bacteria in a 10 L chamber equipped with 4 ionizers and operated for one hour, but we did not use these conditions because we were unable to eliminate the effect of ozone. Instead, we filtered the bacteria onto membrane filters (0.45 μm, containing 200 μl of a 1:100 dilution of LB), and exposed them in a 100 L chamber equipped with an ionizer, and with a fan to provide air flow (1.5 m/s). Under these conditions we found that the bacteria tested were killed, as seen in Fig. 2 (and we also tested for several yeasts including, Candida albicans, Candida vartiovaarai, and Cryotococcus flavus to get the results of 20–30 % of the cells survived after 2 h exposure under these experimental conditions; Additional file 2: Figure S2), The tested bacteria were not killed when the fan was turned off, but as we used the fan alone (no ionizer) mode to compare, it was clear that the ions had specific effects on the bacteria.
Previous studies have demonstrated that negative and positive ions have a bactericidal effect. There are several suggested mechanisms for the action of ionizers: electrical phenomena (ions, electric charge, ozone production, ) and electrostatic repulsion . Our evidence that negative and positive ions generate oxidative stress points to an oxidizing effect. In previous studies, the physical and biological mechanisms underlying the effects of ions remained unclear. To clarify these mechanisms we examined the production of ROS by the ionizer, as well as the effect of the ROS on the bacteria. As shown in Fig. 5, the ionizer did indeed generate ROS, and the ROS caused oxidative damage to the bacterial DNA, as shown by a marked increase in the level of 8-oxo-dG, as well as a pronounced reduction in the activity of aconitase, which is known to be sensitive to ROS . The ROS generated by the ionizer also reduced the adenylate energy charge of the cells, although the AEC was not reduced to the level that leads on its own to cell death. Fumarase activity was not affected by the ionizer (data not shown). These findings indicate that negative and positive ions induce oxidative stress, which causes cell death by oxidative damage to cells.
The newly developed ionizer, which generates a relatively low concentration of ozone, showed a bactericidal effect. The generated negative and positive ions induced oxidative stress on the bacteria during exposure. Our results indicate that negative and positive ions induce oxidative stress, which causes cell death by oxidative damage to cells including damage to DNA. Our findings suggest a potential mechanism of the ions on bactericidal effect.
8-OHdG, 8-hydroxydeoxyguanosine; AEC, adenylate energy charge; DCFH-DA, dichlorodihydrofluorescine diacetate; F-mode, fan only mode; IZ + F + C mode, ionizer, fan and ion capture mode; IZ + F-mode, ionizer and fan mode; ROS, reactive oxygen species
The authors wish to acknowledge the financial support of LG Company.
This study was supported by research grant from LG Company.
Availability of data and materials
The data supporting the conclusion of this article are included within the article and supplementary data.
JSP designed the study and undertook the experiments. KSY participated in the laboratory work and measured the ion and ozone concentrations. BJS develop the ionizer. CSJ as the corresponding author conducted the experiments. All authors have read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
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- Noyce JO, Hughes JF. Bactericidal effects of negative and positive ions generated in nitrogen on Escherichia coli. J Electrostatics. 2002;54(2):179–87.View ArticleGoogle Scholar
- Noyce JO, Hughes JF. Bactericidal effects of negative and positive ions generated in nitrogen on starved Pseudomonas veronii. J Electrostatics. 2003;57(1):49–58.View ArticleGoogle Scholar
- Fletcher LA, Gaunt LF, Beggs CB, Shepherd SJ, Sleigh PA, Noakes CJ, Kerr KG. Bactericidal action of positive and negative ions in air. BMC Microbiol. 2007;7(1):32.View ArticlePubMedPubMed CentralGoogle Scholar
- Shargawi JM, Theaker ED, Drucker DB, MacFarlane T, Duxbury AJ. Sensitivity of Candida albicans to negative air ion streams. J Appl Microbiol. 1999;87(6):889–97.View ArticlePubMedGoogle Scholar
- Marin V, Moretti G, Rassu M. Effects of ionization of the air on some bacterial strains. Ann Ig. 1988;1(6):1491–500.Google Scholar
- Kerr KG, Beggs CB. Air ionisation and colonisation/infection with methicillin-resistant Staphylococcus aureus and Acinetobacter species in an intensive care unity: a reply to Jeffers. Intensive Care Med. 2006;32(9):1439.View ArticleGoogle Scholar
- Shepherd SJ, Beggs CB, Smith CF, Kerr KG, Noakes CJ, Sleigh PA. Effect of negative air ions on the potential for bacterial contamination of plastic medical equipment. BMC Infect Dis. 2010;10(1):92.View ArticlePubMedPubMed CentralGoogle Scholar
- Nishimura H. Analysis of bactericidal material generated by electrical devices advertising bactericidal ability against bacteria on the agar gel plates. Kansenshogaku Zasshi. 2012;86(6):723–33.View ArticlePubMedGoogle Scholar
- Sies H. Oxidative stress: introductory remarks. In: Sies H., editor. Oxidative Stress. London: Academic Press; 1985. p. 1–8.Google Scholar
- Farr SB, Kogoma T. Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol Rev. 1991;55(4):561–85.PubMedPubMed CentralGoogle Scholar
- Yoshikawa T, Naito Y. What is oxidative stress? Jpn Med Assoc J. 2002;45(7):271–6.Google Scholar
- Zakeri F, Hirobe T. A cytogenetic approach to the effects of low levels of ionizing radiations on occupationally exposed individuals. Eur J Radiol. 2010;73(1):191–5.View ArticlePubMedGoogle Scholar
- Schwarzwald R, Monkhouse P, Wolfrum J. Picosecond fluorescence lifetime measurement of the OH radical in an atmospheric pressure flame. Chem Phys Lett. 1987;142(1):15–8.View ArticleGoogle Scholar
- Luts A. Evolution of negative small ions at enhanced ionization. J Geophys Res Atmos. 1995;100(D1):1487–96.View ArticleGoogle Scholar
- Tammet H, Horrak U, Laakso L, Kulmala M. Factors of air ion balance in a coniferous forest according to measurements in Hyytiälä, Finland. Atmos Chem Phys. 2006;6(11):3377–90.View ArticleGoogle Scholar
- Sies H. Biochemistry of oxidative stress. Angew Chem Int Ed Engl. 1986;25(12):1058–71.View ArticleGoogle Scholar
- Gardner PR, Fridovich I. Superoxide sensitivity of the Escherichia coli aconitase. J Biol Chem. 1991;266(29):19328–33.PubMedGoogle Scholar
- Atkinson DE, Walton GM. Adenosine triphosphate conservation in metabolic regulation rat liver citrate cleavage enzyme. J Biol Chem. 1967;242(13):3239–41.PubMedGoogle Scholar
- Chapman AG, Atkinson DE. Stabilization of adenylate energy charge by the adenylate deaminase reaction. J Biol Chem. 1973;248(23):8309–12.PubMedGoogle Scholar
- Chapman AG, Fall L, Atkinson DE. Adenylate energy charge in Escherichia coli during growth and starvation. J Bacteriol. 1971;108(3):1072–86.PubMedPubMed CentralGoogle Scholar
- Varghese S, Tang Y, Imlay JA. Contrasting sensitivities of Escherichia coli aconitases A and B to oxidation and iron depletion. J Bacteriol. 2003;185(1):221–30.View ArticlePubMedPubMed CentralGoogle Scholar