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Fig. 2 | BMC Microbiology

Fig. 2

From: Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety

Fig. 2

Effects of tissue pooling on qPCR results. Upper part shows infection sites on detached maize leaves at 96 hpi and the indicated pooling schemes for DNA extraction. The lower part shows the results of the corresponding qPCRs that used 10 ng of total DNA from each pool. An individual column represents the absolute amount of fungal DNA determined in the leaf disc pool that is boxed with the same colour in the upper part. Grey columns represent the calculated means and standard errors (SE) for data merged from all pools. a Six excised tissue samples were combined from individual leaves. b Excised tissue samples were combined from five different leaves each carrying six infection sites, according to the position of the inoculum on the leaf. c Excised tissue samples were combined from five leaves each carrying a single infection site in its middle

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BMC Microbiology

ISSN: 1471-2180

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