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Table 3 Temperature-dependent resistance of Δhns cells to phage λvir in the presence of phage acquired spacer

From: Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS

strain genotype Plaque forming units (PFUs)
30 °C 37 °C
IIB965 hns + Δcas1 + λT3 4.63 × 1010 ± 1.6 × 1010 5.8 × 1010 ± 1.14 × 1010
IIB966 Δhns Δcas1 + λT3 ~5 × 105 3.35 × 1010 ± 9 × 109
IIB1039 hns + Δcas1 + λc + λT3 3.37 × 1010 ± 2.5 × 109 2.97 × 1010 ± 9.7 × 109
IIB1040 Δhns Δcas1 + λc + λT3 ~ 3 × 102 2 × 1010 ± 6 × 109
  1. Cell lawns of strains hns +cas1) + λc + λT3 and Δhnscas1) + λc + λT3 were infected with phage dilutions (from 100 to 10−8) and incubated at either 30 °C or 37 °C. The average of at least three independent experiments are shown