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Table 3 Temperature-dependent resistance of Δhns cells to phage λvir in the presence of phage acquired spacer

From: Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS

strain

genotype

Plaque forming units (PFUs)

30 °C

37 °C

IIB965

hns + Δcas1 + λT3

4.63 × 1010 ± 1.6 × 1010

5.8 × 1010 ± 1.14 × 1010

IIB966

Δhns Δcas1 + λT3

~5 × 105

3.35 × 1010 ± 9 × 109

IIB1039

hns + Δcas1 + λc + λT3

3.37 × 1010 ± 2.5 × 109

2.97 × 1010 ± 9.7 × 109

IIB1040

Δhns Δcas1 + λc + λT3

~ 3 × 102

2 × 1010 ± 6 × 109

  1. Cell lawns of strains hns + (Δcas1) + λc + λT3 and Δhns (Δcas1) + λc + λT3 were infected with phage dilutions (from 100 to 10−8) and incubated at either 30 °C or 37 °C. The average of at least three independent experiments are shown
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BMC Microbiology

ISSN: 1471-2180

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