Mycobacterium tuberculosis causing tuberculous lymphadenitis in Maputo, Mozambique
- Sofia Omar Viegas1, 2, 3Email author,
- Solomon Ghebremichael4,
- Leguesse Massawo1,
- Matos Alberto5,
- Fabíola Couto Fernandes5, 6,
- Eliane Monteiro5,
- David Couvin7,
- José Maiane Matavele1,
- Nalin Rastogi7,
- Margarida Correia-Neves3, 8, 9,
- Adelina Machado2,
- Carla Carrilho5, 6,
- Ramona Groenheit4,
- Gunilla Källenius3 and
- Tuija Koivula3, 4
© Viegas et al. 2015
Received: 23 June 2015
Accepted: 12 November 2015
Published: 21 November 2015
The zoonosis bovine tuberculosis (TB) is known to be responsible for a considerable proportion of extrapulmonary TB. In Mozambique, bovine TB is a recognised problem in cattle, but little has been done to evaluate how Mycobacterium bovis has contributed to human TB. We here explore the public health risk for bovine TB in Maputo, by characterizing the isolates from tuberculous lymphadenitis (TBLN) cases, a common manifestation of bovine TB in humans, in the Pathology Service of Maputo Central Hospital, in Mozambique, during one year.
Among 110 patients suspected of having TBLN, 49 had a positive culture result. Of those, 48 (98 %) were positive for Mycobacterium tuberculosis complex and one for nontuberculous mycobacteria. Of the 45 isolates analysed by spoligotyping and Mycobacterial Interspersed Repetitive Unit – Variable Number Tandem Repeat (MIRU-VNTR), all were M. tuberculosis. No M. bovis was found.
Cervical TBLN, corresponding to 39 (86.7 %) cases, was the main cause of TBLN and 66.7 % of those where from HIV positive patients.
We found that TBLN in Maputo was caused by a variety of M. tuberculosis strains. The most prevalent lineage was the EAI (n = 19; 43.2 %). Particular common spoligotypes were SIT 48 (EAI1_SOM sublineage), SIT 42 (LAM 9), SIT 1 (Beijing) and SIT53 (T1), similar to findings among pulmonary cases.
M. tuberculosis was the main etiological agent of TBLN in Maputo. M. tuberculosis genotypes were similar to the ones causing pulmonary TB, suggesting that in Maputo, cases of TBLN arise from the same source as pulmonary TB, rather than from an external zoonotic source. Further research is needed on other forms of extrapulmonary TB and in rural areas where there is high prevalence of bovine TB in cattle, to evaluate the risk of transmission of M. bovis from cattle to humans.
Tuberculosis (TB) ranks as the second leading cause of death from a single infectious agent, after the human immunodeficiency virus (HIV) . In 2013, an estimated 9 million people developed TB and 1.5 million died from the disease, from whom 360 000 were HIV positive . The African continent accounts for one quarter of all TB cases in the world and also has the highest rates of cases and deaths relative to population .
Mozambique is one of the high burden TB and HIV countries with a prevalence of HIV infection in adults of 11.5 %  and an estimated TB prevalence of 559 per 100 000 population. Fifty six percent of the TB patients in Mozambique are estimated to be HIV positive . Among all reported TB cases in 2013, 9.8 % were extrapulmonary .
TB is caused by bacteria of the Mycobacterium tuberculosis complex. The Mycobacterium tuberculosis and the Mycobacterium bovis are the primary agents of the disease in humans and cattle respectively.
The TB epidemic in Mozambique is caused by an extensive diversity of M. tuberculosis spoligotypes with predominance of LAM, EAI, T and Beijing lineages . To our knowledge, no information regarding lineages involved in extrapulmonary TB in Mozambique is available.
Bovine TB, caused by M. bovis, is the main zoonotic disease caused by mycobacteria, affecting cattle, other domesticated animals and several free or captive wildlife species. In Mozambiquethe overall prevalence of BTB in cattle is 13.6 % , varying from 0.98 % in Massingir  to 39.6 % in the Govuro district . Little is known about the impact of bovine TB as a human disease in low income countries, particularly in HIV positive patients, including in Mozambique.
In Africa, bovine TB accounts for an estimated median proportion of 2.8 % (range 0 %–37.7 %) of all reported human TB cases .
Bovine TB is spread to humans, typically by ingestion of unpasteurized milk or contaminated meat, causing extrapulmonary TB, but can also be transmitted by inhalation of aerosols causing pulmonary TB [8, 9]. Several studies have detected M. bovis in tuberculous lymphadenitis (TBLN) cases [10–15], being the most common among all extrapulmonary TB cases.
In the present study, we explored the public health risk for bovine TB in Maputo, capital of Mozambique, by characterizing the isolates from TBLN cases, during one year, in the Pathology Service of Maputo Central Hospital.
Demographic data of the 45 patients that were analysed by genotyping methods
Not tested for HIV
Previously TB treatment
Contact with TB patients
The patients’ median age was 33 years (11.1 SD) with a range of 18–75 years. Stratification according to age showed that 39 (86.7 %) of the patients were aged 18–45, while 6 (13.3 %) were above 46 years.
Fifteen (33.3 %) patients had been previously treated for TB and 15 (33.3 %) had had previous contact with TB patients.
Site of sample collection
Cervical lymphadenitis was the main cause of TBLN in Maputo. Of the lymph node samples 39 (86.7 %) were collected from the cervical region, two (4.4 %) from axillary site and one (2.2 %) from inguinal region. Other sites were breast, chest and thigh (one case, 2.2 % from each site).
HIV serology and drug resistance
Among the 45 patients, 30 (66.7 %) were HIV positive (19 males and 11 females), nine (20.0 %) were HIV negative and six (13.3 %) were not tested for HIV. Of the cervical TBLN cases, 26 (66.7 %) were HIV positive patients. Of the 61 patients with negative or contaminated culture results, 39 (63.9 %) were HIV positive, nine (14.8 %) were HIV negative and 13 (21.3 %) were not tested for HIV. No statistical association was found between HIV serology and cervical TBLN.
In Mozambique, being a mine worker is considered a risk factor for HIV , information related to previous work in South African mines was collected; among all positive cultures, four were from mine workers, of them two were HIV positive and two HIV negative.
Culture and cytology results
Among all, 49 patients had a positive mycobacterial culture, giving a culture positivity rate of 44.5 %. Of them, 48 isolates were identified as M. tuberculosis complex and one as nontuberculous mycobacteria (NTM; 32 years, male, HIV positive). From the 59 culture negative patients there were an additional 15 (25.4 %) cases Ziehl Neelsen (ZN) positive on cytology (morphological evidence of mycobacterial infection). In the remaining 44 patients, based on cytology, there was a specific diagnosis other than mycobacterial infection.
Among the 48 culture confirmed TB cases, 45 isolates were analysed by spoligotyping and Mycobacterial Interspersed Repetitive Unit-Variable Number Tandem Repeat (MIRU-VNTR). For the remaining three isolates and for the NTM, DNA was not available, because there was no growth during the re-culture procedure (Fig. 1).
Mixed infection isolate
Spoligotyping is a simple, rapid and cost effective method for simultaneous detection and typing of the M. tuberculosis complex. It is the method of choice for strains with less than five copies of the insertion sequence IS6110, like M. bovis strains, which usually contain only one or two IS6110 copies [17–19]. By spoligotyping, M. tuberculosis isolates are characterized by the absence of spacers 33–36, while M. bovis usually lack spacers 39–43 .
Spoligotyping was performed on 45 isolates. Of them, all were defined as M. tuberculosis and no M. bovis was found.
For each isolate, their binary/octal description, their lineages and SITs are summarized in Table 2. Four SITs (containing seven isolates) were newly created either within the present study or after a match with an orphan in the database. Nine patterns were in clusters, containing 30 isolates (2 to 6 isolates per cluster), amounting to an overall clustering rate of 68.2 % (30/44).
As shown in Table 2, the most common spoligotypes found in this study were SIT48 (East African-Indian_ Somalia; EAI1_SOM) with six isolates; SIT1 (Beijing lineage) and SIT42 (Latin America Mediterranean; LAM 9) with five isolates each and SIT53 (T1) with four isolates. The most common lineage was the EAI (n = 19; 43.2 %).
Figure 2 shows the MIRU-VNTR clusters and respective spoligotyping results obtained in this study. Among the 44 isolates analysed by MIRU-VNTR, a wide variety of patterns were observed. Only three clusters of two isolates each were formed, cluster I (Lineage H3, SIT 4094); cluster II (Beijing lineage, SIT 1); cluster III (Lineage EAI1_SOM, SIT 48). The remaining patterns were unique, i.e., did not cluster with any other isolate within this study.
Minimum Spanning Trees (MST) depicts evolutionary relationships between the M. tuberculosis genotypes in our study using spoligotyping and/or 24-loci MIRU-VNTR typing. The MST based on spoligotypes alone (Additional file 2A) showed isolated relatively well clustered into their respective lineages/sublineages. Isolates belonging to Beijing, EAI lineage and LAM were rather well organized in the three MSTs. However, on the MST based on 24-loci MIRU-VNTR alone (Additional file 2B), one may notice that the isolate represented by SIT254 or 24-MIT Or16 (lineage T5-RUS1), was better correlated with isolates belonging to LAM lineage. Furthermore, the isolate represented by SIT4 or 24-MIT Or30 (Unknown lineage) was also close to LAM lineage strains. Contrary to the MST based on spoligotypes alone (Additional file 2A) and on spoligotypes + 24-loci MIRU-VNTR (Additional file 2C), the MST based on 24-loci MIRU-VNTR alone (Additional file 2B) depicted a similarity between isolates belonging to Beijing lineage and the unique isolate (SIT952 or 24-MIT Or01) representing CAS1-Delhi lineage. For a better data mining of isolates related to 24-loci MIRU-VNTR information, one can refer to Additional file 1 showing the whole picture of association between the various genotypes.
In this study we presented for the first time the lineages of M. tuberculosis complex causing extrapulmonary TB in Maputo, Mozambique. Extrapulmonary TB is reported in 11.6 % of all TB cases in the country , the majority of them being TBLN.
Several studies have shown correlation between HIV infection and TBLN [21–23]. The synergies between TB and HIV infection [24, 25] have resulted in an increase in the incidence of TBLN and have further complicated TB control. In this study, the high prevalence of HIV positive patients (66.7 %) among TBLN cases, might suggest a rising trend of HIV infection associated with TBLN in Maputo. A possible explanation for the high rate of HIV among TBLN cases might be confounders associated with HIV. However, the numbers related to previous mine working did not allow the interpretation of potential associations with HIV and information related to alcohol, drug abuse, ex-imprisonment or smoking was not collected.
Laboratory detection of bovine TB is a challenge, particularly in low income countries. Microscopy for mycobacteria on the FNA is the initial diagnostic procedure for lymphadenitis in Mozambique; although it does not differentiate between M. tuberculosis and M. bovis, it is considered a reliable TBLN diagnostic method, including in HIV positive individuals [26–29]. Molecular typing methods for M. tuberculosis complex detection on FNA specimen are costly and require technical expertise, therefore, are not implemented as a routine method in the country, making the detection of bovine TB difficult.
In this study, among all TBLN suspects, 43.6 % were confirmed to have M. tuberculosis complex strains on culture and one was NTM, no M. bovis was found, showing that M. tuberculosis is the main cause of TBLN in Maputo. The additional 15 (25.4 %) cases that were positive on cytology might be due to infection by either M. tuberculosis complex or NTM which were not detected by culture.
These results are compatible with two studies conducted in the North of Ethiopia, where no M. bovis was detected and M. tuberculosis was identified as the main etiological agent in TBLN cases [30, 31]. On the other hand, another study conducted in Guji zone of Ethiopia, an area inhabited by pastoral and agro-pastoral communities whose livelihood is based on livestock production, among 173 isolates, three were M. bovis; the same study analysed 39 livestock samples, where one M. tuberculosis was isolated in camels, suggesting transmission between livestock and humans in this pastoral area. This last study emphasises the importance of an appropriate study area, where risk factors, including close contact between humans and livestock, and consumption of raw milk and meat by the communities, are present. This can be one of the reasons for the absence of M. bovis in the present study.
By spoligotyping, the main lineages of TBLN were the EAI, Beijing; LAM and T1; and the major SITs, were SIT48, SIT1, SIT42 and SIT53. These genotypes are also predominant in pulmonary cases in Mozambique , indicating that there are no differences in the population of strains in pulmonary and extrapulmonary cases. Similarities between pulmonary and extrapulmonary cases were observed in other countries, i.e. Ethiopia , Thailand , Madagascar  and Brasil .
In African countries, little is known about the common lineages responsible for extrapulmonary TB. A study conducted in the neighbouring South Africa, in children, showed similarities with our findings, stressing the proximity between the two countries. In the South African study, 21.2 % of the M. tuberculosis isolates belonged to the LAM lineage, and 20 % to the Beijing lineages . In another study performed in Ethiopia, spoligotyping revealed that the most common spoligotypes in extrapulmonary TB were SIT54, SIT53, and SIT149 ; SIT54, Lineage T1 was also found to be common in TBLN cases from the present study.
The ancestral EAI lineage, most predominant in this study (42.2 %), and one of the prevalent lineages in pulmonary TB cases in Mozambique, with a predominance of 29.7 % ; is considered to be endemic in the Southern region of India [37, 38]. The migration link between India, particularly South India, and Mozambique arisen since the second half of the 19th century, when Indian traders practised the trade routes of the Indian Ocean, for transnational connections. The high prevalence of EAI lineages in Maputo might represent an indication of TB transmission between the two countries.
The Beijing lineage was also found to be one of the most common lineages in TBLN cases from this study. In South Africa, it was the second most common spoligotype found in extrapulmonary cases  and in Thailand, Beijing lineage was reported to be the most predominant in extrapulmonary TB cases, with 56  and 57.9 % . Further research is needed to evaluate whether Beijing lineage has any particular association with extrapulmonary TB.
We have also shown an association between the Beijing lineage and HIV infection , although in this study, perhaps because of the sample size, it was not possible to find any relationship between a particular lineage and HIV infection. Furthermore, analysis of the spoligotyping lineages did not show any association with a particular clinical expression of the disease (data not shown).
Based on MIRU-VNTR analysis, we could observe a wide diversity of patterns, within strain lineages, showing that TB lymphadenitis in Maputo is not caused by a particular strain but by a wide variety of strains, an indication that risk factors for developing TBLN are rather associated with host than M. tuberculosis strain.
In this study, a mixed M. tuberculosis complex infection was detected in one case of TBLN. Mixed M. tuberculosis infections, a potential obstacle for tuberculosis treatment and control, occurs when an individual is simultaneously infected with more than one strain of M. tuberculosis complex. In high TB prevalence settings, mixed infections are frequent, implying high reinfection rates and the absence of efficient protective immunity conferred by the initial infection .
In pulmonary TB cases from Mozambique, we have shown mixed infection with a Beijing and non-Beijing strain in two out of five Manu strains .
In South Africa, a study conducted in Cape Town, showed that 57 % of patients infected with a Beijing strain were also infected with a non-Beijing strain . Other countries have also reported mixed infection within different strains from the M. tuberculosis complex, i.e. Botswana , China , Taiwan .
In this study, no M. bovis was found. In low income countries there are no effective animal TB control programmes and surveillance, and the epidemiological and public health aspects of infection due to bovine TB are scarce [7–9]. This situation is aggravated by the presence of additional risk factors such as human behaviour and the high prevalence of HIV infections [8, 9, 13]. In African countries, a median of 2.8 % (range 0 %–37.7 %) of all humans cases of TB are estimated to be caused by M. bovis . Variances on prevalence are observed in different sites; those differences might be influenced by sampling, study area and diagnostic methods. Prevalence varies from; 17 and 4.4 % in Ethiopia [12, 46], 16 in Tanzania , 7 in Uganda , 3 in Ghana  and 15.38 % in Nigeria . The genotyping findings from this study and the findings in pulmonary cases  indicate that the overall contribution of M. bovis to human TB in Maputo is minor. However, the present study has certain limitations. The small number of positive cases on culture might have reduced the chances of finding M. bovis as well as the statistical power and have affected the conclusions regarding the significance of the different variables and M. tuberculosis lineages. Furthermore, patients from this study are from urban or peri-urban areas of Maputo where livestock and consumption of unpasteurized milk is minor, thus, exposure to M. bovis is less.
The occurrence of zoonotic TB is greatly dependent on the presence of bovine TB in cattle. M bovis in cattle is very frequent in certain areas of Mozambique; a recent publication has demonstrated a high prevalence of bovine TB in cattle of 39.6 % (95 % CI 36.8–42.5) in one particular district of Mozambique . Another study carried out in 2008 in the same region reported a bovine TB prevalence rate of 61.9 % (95 % CI: 55.8–67.8) . Further research is needed on cases of abdominal TB and other forms of TB, and in pastoral areas, where the prevalence of bovine TB in cattle is known to be high in order to have a better answer about the public health importance of this zoonotic disease in Mozambique.
M. tuberculosis was the main etiological agent of TBLN in Maputo. M. tuberculosis lineages in TBLN were similar to the ones previously reported to cause pulmonary TB, suggesting that in Maputo, cases of TBLN arise from the same source as pulmonary TB, rather than from an external zoonotic source.
Institutional permission to conduct the study was obtained from the National Bioethics Committee of the Ministry of Health in Maputo, Mozambique, reference number 216/CNBS/14. The patients were included after understanding the study and had signed an informed consent.
In Mozambique there are three pathology services, in Maputo, Beira and Nampula, each of them responding for the South, Central and North region of the country respectively.
The Pathology Service of Maputo Central Hospital is the only referral site in Maputo for diagnosing TBLN, patients suspected of mycobacterial infection were referred from different health units for diagnosis. During the study period, they have received 677 patients suspected of TBLN, of whom, 110 (16.2 %) were included in the study. The swellings observed were cervical, axillary or from other sites, either as a unilateral single or multiple mass or masses. Fistula formation could also been seen in certain cases.
Patients who also had pulmonary involvement were considered as extrapulmonary TB in our analysis.
Patients and specimens
This study was conducted from July 2013 to July 2014 at the Pathology Service of Maputo Central Hospital in Maputo, Mozambique. A total of 110 patients with suspected TBLN and subjected to FNA were included in the study. For each patient, a questionnaire was applied.
Demographic and clinical information of the participants was collected by trained clinical nurses using a pre-tested questionnaire. HIV test results were collected from the medical records after obtaining written consent from the patients. Patients without HIV results were advised for testing and that was only performed after patients consent.
Only patients suspected of TBLN that consented and could give at least 0,1 ml of sample were recruited to participate in the study.. That was applied in order to have enough material for the routine smear performed in the unit, direct microscopy using conventional ZN staining and cytology, and subsequent assays to be performed within the study.
All material was collected by a Specialist Physician from the Pathology Service, using the FNA Cytology procedure in use in the Unit, as described below.
Collection of lymph node aspirate and staining
Lymph node aspirate was collected as a routine procedure in the Pathology Service for all suspected of having TBLN. Briefly FNA was performed from a swollen superficial lymph node by using a sterile 23-gauge needle with an attached 10 ml syringe. The overlying area was cleaned with 70 % alcohol. Then the node was punctured by developing a negative pressure in the syringe. Multiple in and out passes were made by the needle without exiting the node. After removing the needle, in all cases, special ZN staining for acid fast bacilli was done and cytology was performed. The remaining aspirated sample was referred to The National TB Reference Laboratory for culture.
Culture and identification methods
The cultures were performed using N-Acetyl-L-Cysteine-Sodium Hydroxide (NALC-4 % NaOH) decontamination method as previously described  and inoculated into three culture media: 1) Liquid culture (BD BBL™ MGIT™ Mycobacteria Growth Indicator Tube), 2) Löwenstein-Jensen slants (BD BBL™ Lowenstein-Jensen Medium) and 3) Stonebrinkslants (in house made-). All tubes were incubated at 37 °C..
All positive cultures were accordingly identified as M. tuberculosis complex or not using SD BIOLINE TB Ag MPT64 rapid test according to manufacturer’s instructions.
Isolates identified as M. tuberculosis complex were subjected to Line Probe Assay, GenoType MTBDRplus (Hain, Nehren, Germany), for detection of MDR TB as previously described .
Standard spoligotyping  was performed generally as described by Kamerbeek and colleagues. Spoligotyping results were analysed and dendograms created using the BioNumerics Software ver. 7.5 (Applied Maths, Kortrijk, Belgium).
Spoligotyping patterns were also compared with the ones existing in the international Spoligotyping database SITVIT2, which is an updated version of SITVITWEB . Shared international types (SITs) that were newly-created either within the present study or after a match with an orphan in the database, were assigned a new SIT number.
Mycobacterial Interspersed Repetitive Unit – Variable Number Tandem Repeat (MIRU-VNTR) analysis
Standardized 24-loci MIRU-VNTR typing  was performed using the MIRU-VNTR typing kit (Genoscreen, Lille, France). The PCR-products were run with 1200 LIZ size standard (GeneScan, Applied Biosystems) on ABI3500 sequencers. Sizing of the PCR-fragments and assignments of MIRU-VNTR alleles were done with the GeneMapper software version 4.1 (Applied Biosystems) according to the manufacturers’ instructions. MIRU-VNTR were also compared with SITVIT2, and newly created 24-loci MIRU-VNTR International Types (24-MITs) were assigned.
HIV testing was performed at the Sanitary Unit of enrolment according to the recommendations by the Ministry of Health, Mozambique. Two rapid HIV tests were used sequentially, Unigold Recombinant HIV (Trinity Biotech, Wicklow, Ireland) and Determine HIV-1/2 (Abbot, Tokyo, Japan). Samples were tested first with Determine and reported only when negative. Positive samples were confirmed with Unigold. All tests were performed and interpreted according to the manufacturer’s instructions.
Statistical analysis was performed on Data Analysis and Statistical Software (STATA), version 13. Descriptive statistics was used for summarizing demographics data. Categorical variables were presented using frequencies and percentages. Bivariate analyses were performed for TB lymphadenitis versus HIV status, HIV status x lineage using chi-square test. Multinomial Logistic regression model were created with TB lymphadenitis as outcome and sex age and HIV status included as predictors. Interactions were tested and all were not statistically significant. Since no interactions were statistically significant, they are not presented.
Phylogenetic relationships were calculated using Multiple-Locus Variable-number tandem repeat Analysis (MLVA) Compare software version 1.03 (Genoscreen and Ridom Bioinformatics). MST were drawn from spoligotyping and 24-loci MIRU-VNTR typing, to better visualize probable relationships and dependencies between isolates. The phylogenetic trees connect each genotype based on degree of changes required to go from one allele to another (the distance numbers are visible on each edge). Solid black line denotes one unique change between two patterns, while solid grey line denotes 2 changes, bold dashed line denotes 3 changes, and thin dotted line represents 4 or more changes. The size of the circle is proportional to the total number of isolates. The colour of the circles indicates the phylogenetic lineage to which the specific pattern belongs. International Types (IT) numbers appear inside nodes. Both SIT and 24-MIT appeared inside nodes of the tree combining spoligotypes and 24-loci MIRU-VNTR.
This study was funded by the Swedish International Development Cooperation Agency / Department for Research Cooperation (Sida/SAREC) through Eduardo Mondlane University and Karolinska Institutet Research and Training (KIRT) collaboration. We thank the staff of the Pathology Service, at Maputo Central Hospital, who assisted with the sample collection; the nurses who assisted in the data collection and the staff of the National Tuberculosis Reference Laboratory, who assisted in sample processing and culture. We also thank Mikael Mansjo from the Public Health Agency of Sweden, for his support on the molecular analysis.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- World Health Organization. Global tuberculosis report 2014. Geneva: World Health Organization; 2014.Google Scholar
- INSIDA 2009, Relatório final Inquérito nacional de prevalência, riscos Comportamentais e Informação sobre o HIV e SIDA em Moçambique [Internet]. Moçambique: Ministério da Saúde, Instituto Nacional de Saúde Maputo, Moçambique; Instituto Nacional de Estatística Maputo, Moçambique; ICF Macro Calverton, MD, EUA; 2010 p. 310. Available from: https://dhsprogram.com/pubs/pdf/AIS8/AIS8.pdf.
- Viegas SO, Machado A, Groenheit R, Ghebremichael S, Pennhag A, Gudo PS, et al. Molecular diversity of Mycobacterium tuberculosis isolates from patients with pulmonary tuberculosis in Mozambique. BMC Microbiol. 2010;10:195.PubMed CentralView ArticlePubMedGoogle Scholar
- Adelina Machado. Mapping of the distribution of Mycobacterium bovis strains involved in bovine tuberculosis in Mozambique, University of Stellembosh. 2015.Google Scholar
- Tanner M, Inlameia O, Michel A, Maxlhuza G, Pondja A, Fafetine J, et al. Bovine Tuberculosis and Brucellosis in Cattle and African Buffalo in the Limpopo National Park. Mozambique: Transbound Emerg Dis; 2014. Jan;n/a – n/a.Google Scholar
- Moiane I, Machado A, Santos N, Nhambir A, Inlamea O, Hattendorf J, et al. Prevalence of bovine tuberculosis and risk factor assessment in cattle in rural livestock areas of Govuro District in the Southeast of Mozambique. PLoS One. 2014;9(3), e91527.PubMed CentralView ArticlePubMedGoogle Scholar
- Müller B, Dürr S, Alonso S, Hattendorf J, Laisse CJM, Parsons SDC, et al. Zoonotic Mycobacterium bovis-induced tuberculosis in humans. Emerg Infect Dis. 2013;19(6):899–908.View ArticlePubMedGoogle Scholar
- Ayele WY, Neill SD, Zinsstag J, Weiss MG, Pavlik I. Bovine tuberculosis: an old disease but a new threat to Africa. Int J Tuberc Lung Dis Off J Int Union Tuberc Lung Dis. 2004;8(8):924–37.Google Scholar
- Cosivi O, Grange JM, Daborn CJ, Raviglione MC, Fujikura T, Cousins D, et al. Zoonotic tuberculosis due to Mycobacterium bovis in developing countries. Emerg Infect Dis. 1998;4(1):59–70.PubMed CentralView ArticlePubMedGoogle Scholar
- Cicero R, Olivera H, Hernández-Solis A, Ramírez-Casanova E, Escobar-Gutiérrez A. Frequency of Mycobacterium bovis as an etiologic agent in extrapulmonary tuberculosis in HIV-positive and -negative Mexican patients. Eur J Clin Microbiol Infect Dis Off Publ Eur Soc Clin Microbiol. 2009;28(5):455–60.View ArticleGoogle Scholar
- Firdessa R, Berg S, Hailu E, Schelling E, Gumi B, Erenso G, et al. Mycobacterial lineages causing pulmonary and extrapulmonary tuberculosis. Ethiopia Emerg Infect Dis. 2013;19(3):460–3.View ArticlePubMedGoogle Scholar
- Gumi B, Schelling E, Berg S, Firdessa R, Erenso G, Mekonnen W, et al. Zoonotic transmission of tuberculosis between pastoralists and their livestock in South-East Ethiopia. EcoHealth. 2012;9(2):139–49.PubMed CentralView ArticlePubMedGoogle Scholar
- Michel AL, Müller B, van Helden PD. Mycobacterium bovis at the animal-human interface: a problem, or not? Vet Microbiol. 2010;140(3–4):371–81.View ArticlePubMedGoogle Scholar
- Oloya J, Opuda-Asibo J, Kazwala R, Demelash AB, Skjerve E, Lund A, et al. Mycobacteria causing human cervical lymphadenitis in pastoral communities in the Karamoja region of Uganda. Epidemiol Infect. 2008;136(5):636–43.PubMed CentralView ArticlePubMedGoogle Scholar
- Popescu MR, Călin G, Strâmbu I, Olaru M, Bălăşoiu M, Huplea V, et al. Lymph node tuberculosis - an attempt of clinico-morphological study and review of the literature. Romanian J Morphol Embryol Rev Roum Morphol Embryol. 2014;55(2 Suppl):553–67.Google Scholar
- Baltazar CS, Horth R, Inguane C, Sathane I, César F, Ricardo H, et al. HIV prevalence and risk behaviors among Mozambicans working in south African mines. AIDS Behav. 2015;19(S1):59–67.PubMed CentralView ArticleGoogle Scholar
- Bauer J, Andersen ÅB, Kremer K, Miörner H. Usefulness of spoligotyping to discriminate IS6110 Low-copy-number mycobacterium tuberculosis complex strains cultured in Denmark. J Clin Microbiol. 1999;37(8):2602–6.PubMed CentralPubMedGoogle Scholar
- Cronin WA, Golub JE, Magder LS, Baruch NG, Lathan MJ, Mukasa LN, et al. Epidemiologic usefulness of spoligotyping for secondary typing of mycobacterium tuberculosis isolates with Low copy numbers of IS6110. J Clin Microbiol. 2001;39(10):3709–11.PubMed CentralView ArticlePubMedGoogle Scholar
- Goyal M, Saunders NA, van Embden JD, Young DB, Shaw RJ. Differentiation of Mycobacterium tuberculosis isolates by spoligotyping and IS6110 restriction fragment length polymorphism. J Clin Microbiol. 1997;35(3):647–51.PubMed CentralPubMedGoogle Scholar
- Van Soolingen D. Molecular epidemiology of tuberculosis and other mycobacterial infections: main methodologies and achievements. J Intern Med. 2001;249(1):1–26.View ArticlePubMedGoogle Scholar
- Bem C, Patil PS, Bharucha H, Namaambo K, Luo N. Importance of human immunodeficiency virus-associated lymphadenopathy and tuberculous lymphadenitis in patients undergoing lymph node biopsy in Zambia. Br J Surg. 1996;83(1):75–8.View ArticlePubMedGoogle Scholar
- Bezabih M, Abdissa A, Gadisa E, Aseffa A. Patterns of enlarged cervical lymph nodes among HIV positive and negative patients in southwestern Ethiopia: a cytopathlogic analysis. Ethiop Med J. 2014;52(1):19–25.PubMedGoogle Scholar
- Sibanda EN, Stanczuk G. Lymph node pathology in Zimbabwe: a review of2194 specimens. QJM Int J Med. 1993;86(12):811–7.Google Scholar
- Bruchfeld J, Correia-Neves M, Källenius G. Tuberculosis and HIV Coinfection, Cold Spring Harb Perspect Med. 2015. Feb 26.Google Scholar
- Ronacher K, Joosten SA, van Crevel R, Dockrell HM, Walzl G, Ottenhoff THM. Acquired immunodeficiencies and tuberculosis: focus on HIV/AIDS and diabetes mellitus. Immunol Rev. 2015;264(1):121–37.View ArticlePubMedGoogle Scholar
- Saikia UN, Dey P, Jindal B, Saikia B. Fine needle aspiration cytology in lymphadenopathy of HIV-positive cases. Acta Cytol. 2001;45(4):589–92.View ArticlePubMedGoogle Scholar
- Sarma PK, Chowhan AK, Agrawal V, Agarwal V. Fine needle aspiration cytology in HIV-related lymphadenopathy: experience at a single centre in north India. Cytopathol Off J Br Soc Clin Cytol. 2010;21(4):234–9.View ArticleGoogle Scholar
- Shenoy R, Kapadi SN, Pai KP, Kini H, Mallya S, Khadilkar UN, et al. Fine needle aspiration diagnosis in HIV-related lymphadenopathy in Mangalore. India Acta Cytol. 2002;46(1):35–9.View ArticlePubMedGoogle Scholar
- Tirumalasetti N, Prema LP. Lymph nodes cytology in HIV seropositive cases with haematological alterations. Indian J Med Res. 2014;139(2):301–7.PubMed CentralPubMedGoogle Scholar
- Biadglegne F, Tesfaye W, Sack U, Rodloff AC. Tuberculous lymphadenitis in northern Ethiopia: in a public health and microbiological perspectives. PLoS ONE [Internet]. 2013;8:12. Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3857213/.Google Scholar
- Beyene D, Bergval I, Hailu E, Ashenafi S, Yamuah L, Aseffa A, et al. Identification and genotyping of the etiological agent of tuberculous lymphadenitis in Ethiopia. J Infect Dev Ctries. 2009;3(6):412–9.View ArticlePubMedGoogle Scholar
- Srilohasin P, Chaiprasert A, Tokunaga K, Nishida N, Prammananan T, Smittipat N, et al. Genetic diversity and dynamic distribution of mycobacterium tuberculosis isolates causing pulmonary and extrapulmonary tuberculosis in Thailand. J Clin Microbiol. 2014;52(12):4267–74.PubMed CentralView ArticlePubMedGoogle Scholar
- Rasolofo Razanamparany V, Ménard D, Aurégan G, Gicquel B, Chanteau S. Extrapulmonary and pulmonary tuberculosis in Antananarivo (Madagascar): high clustering rate in female patients. J Clin Microbiol. 2002;40(11):3964–9.PubMed CentralView ArticlePubMedGoogle Scholar
- Gomes T, Vinhas SA, Reis-Santos B, Palaci M, Peres RL, Aguiar PP. Extrapulmonary tuberculosis: mycobacterium tuberculosis strains and host risk factors in a large urban setting in brazil. PLoS ONE [Internet]. 2013;8:10. Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3788772/.Google Scholar
- Nicol MP, Sola C, February B, Rastogi N, Steyn L, Wilkinson RJ. Distribution of strain families of mycobacterium tuberculosis causing pulmonary and extrapulmonary disease in hospitalized children in cape town. South Africa J Clin Microbiol. 2005;43(11):5779–81.View ArticlePubMedGoogle Scholar
- Garedew L, Mihret A, Ameni G. Molecular typing of mycobacteria isolated from extrapulmonary tuberculosis patients at Debre Birhan Referral Hospital, central Ethiopia. Scand J Infect Dis. 2013;45(7):512–8.View ArticlePubMedGoogle Scholar
- Narayanan S, Gagneux S, Hari L, Tsolaki AG, Rajasekhar S, Narayanan PR, et al. Genomic interrogation of ancestral Mycobacterium tuberculosis from south India. Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis. 2008;8(4):474–83.View ArticleGoogle Scholar
- Singh J, Sankar MM, Kumar P, Couvin D, Rastogi N, Singh S, et al. Genetic diversity and drug susceptibility profile of Mycobacterium tuberculosis isolated from different regions of India. J Infect. 2015;29.Google Scholar
- Faksri K, Drobniewski F, Nikolayevskyy V, Brown T, Prammananan T, Palittapongarnpim P, et al. Epidemiological trends and clinical comparisons of Mycobacterium tuberculosis lineages in Thai TB meningitis. Tuberc Edinb Scotl. 2011;91(6):594–600.View ArticleGoogle Scholar
- Yorsangsukkamol J, Chaiprasert A, Prammananan T, Palittapongarnpim P, Limsoontarakul S, Prayoonwiwat N. Molecular analysis of Mycobacterium tuberculosis from tuberculous meningitis patients in Thailand. Tuberc Edinb Scotl. 2009;89(4):304–9.View ArticleGoogle Scholar
- Viegas SO, Machado A, Groenheit R, Ghebremichael S, Pennhag A, Gudo PS, et al. Mycobacterium tuberculosis Beijing genotype is associated with HIV infection in Mozambique. PLoS One. 2013;8(8), e71999.PubMed CentralView ArticlePubMedGoogle Scholar
- Warren RM, Victor TC, Streicher EM, Richardson M, Beyers N, Gey van Pittius NC, et al. Patients with active tuberculosis often have different strains in the same sputum specimen. Am J Respir Crit Care Med. 2004;169(5):610–4.View ArticlePubMedGoogle Scholar
- Zetola NM, Shin SS, Tumedi KA, Moeti K, Ncube R, Nicol M, et al. Mixed mycobacterium tuberculosis complex infections and false-negative results for rifampin resistance by GeneXpert MTB/RIF Are associated with poor clinical outcomes. J Clin Microbiol. 2014;52(7):2422–9.PubMed CentralView ArticlePubMedGoogle Scholar
- Pang Y, Zhou Y, Wang S, Song Y, Ou X, Zhao B, et al. Prevalence and risk factors of mixed Mycobacterium tuberculosis complex infections in China. J Infect. 2015;29.Google Scholar
- Wang J-Y, Hsu H-L, Yu M-C, Chiang C-Y, Yu F-L, Yu C-J, et al. Mixed infection with Beijing and non-Beijing strains in pulmonary tuberculosis in Taiwan: prevalence, risk factors, and dominant strain. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis. 2011;17(8):1239–45.Google Scholar
- Kidane D, Olobo JO, Habte A, Negesse Y, Aseffa A, Abate G, et al. Identification of the causative organism of tuberculous lymphadenitis in ethiopia by PCR. J Clin Microbiol. 2002;40(11):4230–4.PubMed CentralView ArticlePubMedGoogle Scholar
- Kazwala RR, Daborn CJ, Sharp JM, Kambarage DM, Jiwa SF, Mbembati NA. Isolation of Mycobacterium bovis from human cases of cervical adenitis in Tanzania: a cause for concern? Int J Tuberc Lung Dis Off J Int Union Tuberc Lung Dis. 2001;5(1):87–91.Google Scholar
- Addo K, Owusu-Darko K, Yeboah-Manu D, Caulley P, Minamikawa M, Bonsu F, et al. Mycobacterial species causing pulmonary tuberculosis at the korle bu teaching hospital, accra, ghana. Ghana Med J. 2007;41(2):52–7.PubMed CentralPubMedGoogle Scholar
- Mawak J, Gomwalk N, Bello C, Kandakai-Olukemi Y. Human pulmonary infections with bovine and environment (atypical) mycobacteria in jos. Nigeria Ghana Med J. 2006;40(4):132–6.PubMedGoogle Scholar
- Macucule B. Study of the prevalence of bovine tuberculosis in Govuro District. [University of Pretoria, South Africa]: Inhambane Province, Mozambique [MSc]; 2008.Google Scholar
- Kubica GP, Dye WE, Cohn ML, Middlebrook G. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am Rev Respir Dis. 1963;87:775–9.PubMedGoogle Scholar
- Vijdea R, Stegger M, Sosnovskaja A, Andersen AB, Thomsen VØ, Bang D. Multidrug-resistant tuberculosis: rapid detection of resistance to rifampin and high or low levels of isoniazid in clinical specimens and isolates. Eur J Clin Microbiol Infect Dis. 2008;27(11):1079–86.View ArticlePubMedGoogle Scholar
- Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35(4):907–14.PubMed CentralPubMedGoogle Scholar
- Demay C, Liens B, Burguière T, Hill V, Couvin D, Millet J, et al. SITVITWEB--a publicly available international multimarker database for studying Mycobacterium tuberculosis genetic diversity and molecular epidemiology. Infect Genet Evol J Mol Epidemiol Evol Genet Infect Dis. 2012;12(4):755–66.View ArticleGoogle Scholar
- Supply P, Allix C, Lesjean S, Cardoso-Oelemann M, Rüsch-Gerdes S, Willery E, et al. Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of mycobacterium tuberculosis. J Clin Microbiol. 2006;44(12):4498–510.PubMed CentralView ArticlePubMedGoogle Scholar