Table 2 Strains and plasmids used in this study
Genotype and/or characteristics | Reference | |
|---|---|---|
Strains | ||
“Aromatoleum aromaticum” EbN1 | Wild type | [3] |
EbN1-RR001 (ΔetpR mutant) | ΔetpR | This study |
EbN1-RR002 (etpR-complemented mutant) | ΔetpR, pBBR1MCS-2 ΩetpR | This study |
EbN1-RR003 (Wild type containing pBBR1MCS-2) | Wild type, pBBR1MCS-2 | This study |
EbN1-RR004 (ΔetpR mutant containing pBBR1MCS-2) | ΔetpR, pBBR1MCS-2 | This study |
Escherichia coli S17-1 | Pro, thi, hsdR, Tra +, recA−, Trr, Smr, ΩRP4-TE::Mu-Kn::Tn7 | [45] |
Plasmids | ||
pK19mobsacB | KmR, sacB modified from B. subtilis, lacZα | [46] |
pK19 ΩacsA | KmR, sacB modified from B. subtilis, lacZα, acsA from "A. aromaticum" EbN1 | This study |
pK19 ΩacsAebA326/7 | KmR, sacB modified from B. subtilis, lacZα; acsA, ebA326 and part of ebA327 from "A. aromaticum" EbN1 | This study |
pBBR1MCS-2 | KmR, mob, lacZα | [40] |
pBBR1MCS-2 ΩetpR | KmR, mob, lacZα, etpR from "A. aromaticum" EbN1 | This study |