Table 1 Strains and plasmids used in this work

P. syringae pv. tomato

 DC3000

Rif^r

[14]

 ∆iaaL

Km^r

This work

 ∆matE

Km^r

This work

 ∆hrpL

Km^r

This work

E. coli

  E. coli DH5α

F-, ϕ80dlacZ M15, (lacZYA-argF) U169, deoR, recA1, endA,

[66]

hsdR17 (rk ⁻ mk ⁻), phoA, supE44, thi-1, gyrA96, relA1.

Plasmids

 pGEM-T Easy Vector

Amp^r, ori f1, lacZ

Promega, USA

 pGEM-T Vector

Amp^r, ori f1, lacZ

Promega, USA

 pGEM-T-KmFRT-BamHI

Contains Km^r from pKD4 (Amp^r Km^r)

[67]

 pMP220

Expression Vector, Tc^r

[35]

 pJB3Tc20

Expression Vector, Tc^r

[68]

 pIAC1

pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene (Amp^r)

This work

 pIAC2

pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene (Amp^r)

This work

 pIAC3

pGEM-T derivative, contains approx. 0.5 kb on each side of the mat gene (Amp^r)

This work

 pIAC1-Km

pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene interrupted by the kanamycin resistance gene nptII (Amp ^r, Km ^r)

This work

 pIAC2-Km

pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene interrupted by the kanamycin resistance gene nptII (Amp^r, Km^r)

This work

 pIAC3-Km

pGEM-T derivative, contains approx. 0.5 kb on each side of the matE gene interrupted by the kanamycin resistance gene nptII (Amp^r, Km^r)

This work

 pMP220-PmatE

pMP220 derivative, contains the lacZ gene expressed from the P_matE promoter (Tc^r)

This work

 pMP220-R2

pMP220 derivative, contains the lacZ gene expressed from the P_iaaL promoter (Tc^r)

This work

 pJB3-matE

pJB3 derivative, contains the matE gene with RBS

This work

 pJB3-iaaL

pJB3 derivative, contains the iaaL gene with RBS

This work