|
P. syringae pv. tomato
| | |
|
DC3000
|
Rifr
|
[14]
|
|
∆iaaL
|
Kmr
|
This work
|
|
∆matE
|
Kmr
|
This work
|
|
∆hrpL
|
Kmr
|
This work
|
|
E. coli
| | |
|
E. coli DH5α
|
F-, ϕ80dlacZ M15, (lacZYA-argF) U169, deoR, recA1, endA,
|
[66]
|
|
hsdR17 (rk
−
mk
−), phoA, supE44, thi-1, gyrA96, relA1.
| |
|
Plasmids
| | |
|
pGEM-T Easy Vector
|
Ampr, ori f1, lacZ
|
Promega, USA
|
|
pGEM-T Vector
|
Ampr, ori f1, lacZ
|
Promega, USA
|
|
pGEM-T-KmFRT-BamHI
|
Contains Kmr from pKD4 (Ampr Kmr)
|
[67]
|
|
pMP220
|
Expression Vector, Tcr
|
[35]
|
|
pJB3Tc20
|
Expression Vector, Tcr
|
[68]
|
|
pIAC1
|
pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene (Ampr)
|
This work
|
|
pIAC2
|
pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene (Ampr)
|
This work
|
|
pIAC3
|
pGEM-T derivative, contains approx. 0.5 kb on each side of the mat gene (Ampr)
|
This work
|
|
pIAC1-Km
|
pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene interrupted by the kanamycin resistance gene nptII (Amp
r, Km
r)
|
This work
|
|
pIAC2-Km
|
pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene interrupted by the kanamycin resistance gene nptII (Ampr, Kmr)
|
This work
|
|
pIAC3-Km
|
pGEM-T derivative, contains approx. 0.5 kb on each side of the matE gene interrupted by the kanamycin resistance gene nptII (Ampr, Kmr)
|
This work
|
|
pMP220-PmatE
|
pMP220 derivative, contains the lacZ gene expressed from the PmatE promoter (Tcr)
|
This work
|
|
pMP220-R2
|
pMP220 derivative, contains the lacZ gene expressed from the PiaaL promoter (Tcr)
|
This work
|
|
pJB3-matE
|
pJB3 derivative, contains the matE gene with RBS
|
This work
|
|
pJB3-iaaL
|
pJB3 derivative, contains the iaaL gene with RBS
|
This work
|
