Contribution of the non-effector members of the HrpL regulon, iaaL and matE , to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants

Melissa G. Castillo-Lizardo; Isabel M. Aragón; Vivian Carvajal; Isabel M. Matas; María Luisa Pérez-Bueno; María-Trinidad Gallegos; Matilde Barón; Cayo Ramos

doi:10.1186/s12866-015-0503-8

Table 1 Strains and plasmids used in this work

From: Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants

Strain/Plasmid	Genotype^a	Reference
P. syringae pv. tomato
DC3000	Rif^r	[14]
∆iaaL	Km^r	This work
∆matE	Km^r	This work
∆hrpL	Km^r	This work
E. coli
E. coli DH5α	F-, ϕ80dlacZ M15, (lacZYA-argF) U169, deoR, recA1, endA,	[66]
E. coli DH5α	hsdR17 (rk ⁻ mk ⁻), phoA, supE44, thi-1, gyrA96, relA1.
Plasmids
pGEM-T Easy Vector	Amp^r, ori f1, lacZ	Promega, USA
pGEM-T Vector	Amp^r, ori f1, lacZ	Promega, USA
pGEM-T-KmFRT-BamHI	Contains Km^r from pKD4 (Amp^r Km^r)	[67]
pMP220	Expression Vector, Tc^r	[35]
pJB3Tc20	Expression Vector, Tc^r	[68]
pIAC1	pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene (Amp^r)	This work
pIAC2	pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene (Amp^r)	This work
pIAC3	pGEM-T derivative, contains approx. 0.5 kb on each side of the mat gene (Amp^r)	This work
pIAC1-Km	pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene interrupted by the kanamycin resistance gene nptII (Amp ^r, Km ^r)	This work
pIAC2-Km	pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene interrupted by the kanamycin resistance gene nptII (Amp^r, Km^r)	This work
pIAC3-Km	pGEM-T derivative, contains approx. 0.5 kb on each side of the matE gene interrupted by the kanamycin resistance gene nptII (Amp^r, Km^r)	This work
pMP220-PmatE	pMP220 derivative, contains the lacZ gene expressed from the P_matE promoter (Tc^r)	This work
pMP220-R2	pMP220 derivative, contains the lacZ gene expressed from the P_iaaL promoter (Tc^r)	This work
pJB3-matE	pJB3 derivative, contains the matE gene with RBS	This work
pJB3-iaaL	pJB3 derivative, contains the iaaL gene with RBS	This work

^aAmp^r, Km^r, Rif^r and Tc^r indicate resistance to ampicillin, kanamycin, rifampicin and tetracycline, respectively

Back to article page