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Table 1 Strains and plasmids used in this work

From: Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants

Strain/Plasmid Genotypea Reference
P. syringae pv. tomato   
 DC3000 Rifr [14]
 ∆iaaL Kmr This work
 ∆matE Kmr This work
 ∆hrpL Kmr This work
E. coli   
E. coli DH5α F-, ϕ80dlacZ M15, (lacZYA-argF) U169, deoR, recA1, endA, [66]
hsdR17 (rk mk ), phoA, supE44, thi-1, gyrA96, relA1.  
Plasmids   
 pGEM-T Easy Vector Ampr, ori f1, lacZ Promega, USA
 pGEM-T Vector Ampr, ori f1, lacZ Promega, USA
 pGEM-T-KmFRT-BamHI Contains Kmr from pKD4 (Ampr Kmr) [67]
 pMP220 Expression Vector, Tcr [35]
 pJB3Tc20 Expression Vector, Tcr [68]
 pIAC1 pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene (Ampr) This work
 pIAC2 pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene (Ampr) This work
 pIAC3 pGEM-T derivative, contains approx. 0.5 kb on each side of the mat gene (Ampr) This work
 pIAC1-Km pGEM-T derivative, contains approx. 0.5 kb on each side of the iaaL gene interrupted by the kanamycin resistance gene nptII (Amp r, Km r) This work
 pIAC2-Km pGEM-T derivative, contains approx. 0.5 kb on each side of the hrpL gene interrupted by the kanamycin resistance gene nptII (Ampr, Kmr) This work
 pIAC3-Km pGEM-T derivative, contains approx. 0.5 kb on each side of the matE gene interrupted by the kanamycin resistance gene nptII (Ampr, Kmr) This work
 pMP220-PmatE pMP220 derivative, contains the lacZ gene expressed from the PmatE promoter (Tcr) This work
 pMP220-R2 pMP220 derivative, contains the lacZ gene expressed from the PiaaL promoter (Tcr) This work
 pJB3-matE pJB3 derivative, contains the matE gene with RBS This work
 pJB3-iaaL pJB3 derivative, contains the iaaL gene with RBS This work
  1. aAmpr, Kmr, Rifr and Tcr indicate resistance to ampicillin, kanamycin, rifampicin and tetracycline, respectively