Reference genealogy of A. butzleri isolates (n=156). Clusters were calculated by simple matching comparison of 72 accessory genes using pairwise coefficients and UPGMA analysis. The scale represents fingerprint similarity based on the total number of shared loci between isolate profiles and the total number of loci in the assay. Dashed grey line represents a 90% similarity threshold used for clade definition. Isolates sequenced as part of this study are highlighted in yellow; ID 17 (strain L353, PRJNA233527), ID 40 (strain L355, PRJNA233527), ID 64 (strain L348, PRJNA233527), ID 69 (strain L352, PRJNA233527), ID 82 (strain L354, PRJNA233527), ID 100 (strain L349, PRJNA233527), ID 149 (strain L351, PRJNA233527), ID 151 (strain L350, PRJNA233527). Published reference A. butzleri strains are designated with arrows and include ID 68 (strain 7h1h, PRJNA200766), ID 109 (strain JV22, PRJNA61483), ID 138 (strain RM4018, PRJNA58557), ID 154 (strain ED-1, PRJNA158699).