Table 7 Genes differentially expressed in Lactobacillus ruminis DPC 6832
From: Lactobacillus ruminis strains cluster according to their mammalian gut source
Primer pair | DPC 6832 | Function | |||||||
|---|---|---|---|---|---|---|---|---|---|
ID | RNA-seq | RT-PCR | |||||||
Swimming vs. Stationary log2 fold change a | pval | Swarming vs. Stationary log2 fold change b | pval | Fold change | 2ΔΔCT fold change swimming vs. Stationary c | 2ΔΔCT fold change swarming vs. Stationary d | |||
MMOD 1 | LRN_87 | −3.36 | ** | 1.81 | >0.05 | 36 | 12.92 (9.72-17.18) | 105.18 (95.45-115.89) | hypothetical protein |
MMOD 2 | LRN_108 | −5.11 | *** | 2.66 | * | 218 | 2.80 (2.40-3.24) | 4.34 (3.21-5.87) | DeoR family transcriptional regulator |
MMOD 3 | LRN_109 | −3.94 | ** | 3.62 | ** | 189 | 0.06 (0.06-0.07 | 7.80 (6.01-10.13) | 1-phosphofructokinase |
MMOD 4 | LRN_110 | −2.93 | * | 4.29 | ** | 149 | 0.02 (0.02-0.03) | 6.06 (4.40-8.35) | PTS system fructose-specific |
MMOD 5 | LRN_324 | 2.50 | * | −0.86 | >0.05 | 10 | 0.97 (0.74-1.27) | 0.11 (0.08-0.14) | hypothetical protein |
MMOD 6 | LRN_409 | −4.62 | *** | 2.28 | >0.05 | 120 | 0.03 (0.02-0.04) | 1.03 (0.81-1.29) | hypothetical_protein |
MMOD 7 | LRN_520 | −0.74 | >0.05 | 3.39 | ** | 18 | 0.49 (0.36-0.68) | 7.35 (5.57-9.71) | beta-fructofuranosidase |
MMOD 8 | LRN_521 | 0.06 | >0.05 | 4.49 | ** | 22 | 0.16 (0.12-0.21) | 6.07 (4.61-7.98) | MFS Transporter Beta fructofuranosidase |
MMOD 9 | LRN_561 | 1.83 | >0.05 | −1.15 | >0.05 | 8 | 0.58 (0.43-0.77) | 0.12 (0.09-0.15) | hypothetical protein |
MMOD 10 | LRN_598 | 1.24 | >0.05 | 0.67 | >0.05 | 1.48 | 0.24 (0.18-0.33) | 0.46 (0.35-0.62) | flagellin |
MMOD 11 | LRN_933 | 5.15 | *** | 3.24 | * | 4 | 1.42 (1.20-1.70) | 0.72 (0.53-0.98) | hypothetical_protein |
MMOD 12 | LRN_1405/1777 | 1.70 | >0.05 | 2.89 | * | 2.28 | 0.46 (0.33-0.62) | 2.09 (1.54-2.84) | flagellin |
MMOD 13 | LRN_1410 | 1.81 | >0.05 | 3.04 | * | 2.36 | 0.3 (0.27-0.32) | 1.78 (1.31-2.42) | flagellin |
MMOD 14 | LRN_1655 | 0.94 | >0.05 | 1.84 | >0.05 | 1.87 | 0.23 (0.18-0.29) | 0.27 (0.20-0.36) | PTS system sucrose-specific transporter subunit IIABC |