Biofilm formation assessment in Sinorhizobium meliloti reveals interlinked control with surface motility

BMC Microbiology

Table 1 Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristics ^a	Source or reference
Escherichia coli
S17.1	thi, pro, recA, hsdR, hsdM, Rp4Tc::Mu, Km::Tn7; Tp^r, Sm^r, Sp^r	[63]
Sinorhizobium meliloti
GR4	Wild-type	[64]
GR4FDCSS	GR4 (ΔfadD::SmSp), Sm^r Sp^r	This study
Rm1021	SU47 expR102::ISRm2011-1, Sm^r	[65]
1021FDCSS	Rm1021 (ΔfadD::SmSp), Sm^r Sp^r	[25]
1021rhbA	Rm1021 rhbA::Tn5lac; Sm^r Nm^r	[26]
G212rirA	Rm1021 (lac⁻, rirA::Km), Sm^r Km^r	O’Connell, M.
Plasmids
pHC60	IncP plasmid constitutively expressing GFP, Tc^r	[62]

^aTp^r, Sm^r, Sp^r, Nm^r, Km^r, Tc^r: abbreviations for resistance to trimethoprim, streptomycin, spectinomycin, neomycin, kanamycin, and tetracycline.

ISSN: 1471-2180