Figure 5

Qualitative Western blot analysis of extra-cellular Lsc in cell-free supernatant of PG4180 wild type (WT) and its hexR mutant. Cultures are grown in HS medium supplemented with arabinose as sole carbon source at 18°C. 0.5, 2.5, 5 μg of protein samples per lane were electrophoretically separated, transferred to a polyvinylidene fluoride membrane, and hybridized with Lsc-specific polyclonal antibodies.