Table 2 Plasmids used in this study
Plasmid | Relevant genetic markers, features, and/or description | Construction, source, or reference |
|---|---|---|
pBBR1 MCS-2 | Broad-host-range vector, KmR | [57] |
pCM130 | RK2-derived broad-host-range vector with E. coli rrnB terminator preceding polylinker, TcR | [58] |
pJQ200sk | Counter-selectable vector for allelic replacement, sacB, GmR | [59] |
pRVMCS-5 | RK2-derived broad-host-range vector with C. crescentus P van promoter, TcR | [14] |
pVCHYN-5 | Narrow-host-range vector with C. crescentus P van promoter and mCherry, TcR | [14] |
pVO155 | pUC119-derived suicide vector with uidA cassette, NmR/KmR ApR | [60] |
pJC445 | pVO155-P araA , for fusing uidA to sequence upstream of araA | This study |
pJC446 | pVO155-P tauA , for fusing uidA to sequence upstream of tauA | This study |
pJC447 | pVO155-P rhaR , for fusing uidA to sequence upstream of rhaR | This study |
pJC455 | pVO155-P melA , for fusing uidA to sequence upstream of melA | This study |
pJC468 | pVO155-P tauA -pleC, for integrating into chromosome and placing pleC under control of P tauA | This study |
pJC470 | pVO155-P tauA -tatAB, for integrating into chromosome and placing tatB and tatC under control of P tauA | This study |
pJC472 | pCM130-P tauA | This study |
pJC473 | pCM130-tauR-P tauA | This study |
pJC474 | pRVMCS-5-derived plasmid carrying P tauA | This study |
pJC475 | pRVMCS-5-derived plasmid carrying tauR-P tauA | This study |
pJC478 | pCM130-P tauA -uidA | This study |
pJC479 | pCM130-tauR-P tauA -uidA | This study |
pJC503 | pRVMCS-5-derived plasmid carrying tauR-P tauA -mCherry | This study |
pJC507 | pJQ200sk-ΔtauR | This study |
pJC508 | pJQ200sk-ΔtauC | This study |
pJC509 | pJQ200sk-ΔtauY | This study |
pJC514 | pBBR1 MCS-2-tauR-P tauA -mCherry | This study |
pJC538 | pCM130-P tauA -tauC | This study |
pJC539 | pCM130-P tauA -tauY | This study |