Table 1 Strains used in this study
Strain | Relevant genetic markers, features, and/or description | Construction, source, or reference |
|---|---|---|
DH5α | Escherichia coli cloning strain: F − φ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 hsd R17 (rk-, mk+) deoR thi-1 supE44 gyrA96 relA1 | Invitrogen |
DH10B | Escherichia coli cloning strain: F − mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL | Invitrogen |
MT616 | MT607/pRK600; Escherichia coli helper strain for mobilizing RK2/RP4-derived plasmids | [53] |
NA1000 | Caulobacter crescentus syn-1000; previously called CB15N, a synchronizable derivative of wild-type CB15 | [54] |
ZM4 | Zymomonas mobilis wild-type strain, previously called CP4 | [55] |
Rm1021 | Sinorhizobium meliloti SU47 derivative, SmR (progenitor of strains listed below) | [56] |
JOE3273 | SMb20895::pJC445 (P araA -uidA) | Mate pJC445 into Rm1021, select for NmR |
JOE3275 | SMb21526::pJC446 (P tauA -uidA) | Mate pJC446 into Rm1021, select for NmR |
JOE3277 | SMc02323::pJC447 (P rhaR -uidA) | Mate pJC447 into Rm1021, select for NmR |
JOE3334 | SMb21648::pJC455 (P melA -uidA) | Mate pJC455 into Rm1021, select for NmR |
JOE3601 | SMc02369::pJC468 (P tauA -pleC) | Mate pJC468 into Rm1021, select for NmR |
JOE3604 | SMc02066::pJC470 (P tauA -tatAB) | Mate pJC470 into Rm1021, select for NmR |
JOE3844 | ΔtauR (ΔSMb21525) | Allelic replacement using pJC507 |
JOE3846 | ΔtauC (ΔSMb21528) | Allelic replacement using pJC508 |
JOE3848 | ΔtauY (ΔSMb21529) | Allelic replacement using pJC509 |