Identification of glycoproteins secreted by wild-type Botrytis cinerea and by protein O-mannosyltransferase mutants

Table 1 Abundant proteins identified by LC-MS/MS in wild-type B. cinerea and the three Δ bcpmt mutants

Protein name¹- Gene ID	Spectral counts²
Protein name¹- Gene ID	B05	Δbcpmt1	Δbcpmt2	Δbcpmt4
1,3-beta-glucanosyltransferase Glycolipid anchored surface protein - B0510_3559	16	22	21	25
Alpha-amilase (GH13) - BC1G_02623.1	13	12	5	23
Beta-1,3-endoglucanase with a GPI anchor (GH17) - B0510_9551	5	15	6	17
Glucoamylase (CBM20, GH15) - B0510_2137	137	152	52	201
Glucoamylase (CBM20, GH15) - B0510_2884	30	39	15	34
Oxidorreductase - B0510_547	9	20	15	13
Pectin methylesterase BcPME2 (CE8) - B0510_344	31	37	9	4
Phospholipase C - B0510_184	11	17	14	15
Protease (Merops A1) - B0510_6952	7	18	8	12
Pro-Xaa carboxypeptidase (Merops S28) - BC1G_09564.1/BC1G_09565.1	16	23	7	15
Unknown, similar to phytase - B0510_5392	12	17	14	20
Unknown, similar to subtilase family protein - B0510_6786	21	22	4	14
Unknown, similar to yeast spore wall proteins - BC1G_10630.1	8	12	6	15

¹Protein family and/or conserved domains found in sequences are included between brackets where possible: glycosyl hydrolase family (GH…), Merops family (Merops…), carbohydrate esterase family (CE…), or carbohydrate binding modules (CBM…).
²Only proteins fulfilling the following two conditions are displayed: they have at least 15 spectral counts in one of the four strains, and they account for more than 2% of all spectral counts in one of the strains.

ISSN: 1471-2180