Table 1 Plasmids and primers used in the present study
From: Characterization of the hupSL promoter activity in Nostoc punctiformeATCC 29133
Plasmids | Reporter gene | Reference |
|---|---|---|
pSUN202 | gfp | (2) |
pA-gfp | gfp | This study |
pB-gfp | gfp | This study |
pC-gfp | gfp | This study |
pD-gfp | gfp | This study |
pE-gfp | gfp | This study |
pPprbcL-gfp | gfp | This study |
pLR1 | luxAB | P Lindberg, unpublished |
pA-lux | luxAB | This study |
pB-lux | luxAB | This study |
pC-lux | luxAB | This study |
pD-lux | luxAB | This study |
pE-lux | luxAB | This study |
pPprbc-Llux | luxAB | This study |
Primers | Sequence 5'-3' | Restriction site |
GFP | ||
CLONING | ||
A GFP forward | CGCGGTACCAGGCTTCGAGTCCTTTAGGC | KpnI |
B GFP forward | CGCGGTACCTCAATCCCCTAAATTG | KpnI |
C GFP forward | CGCGGTACCTTCTAAAATTCTAGGGGGAAATTG | KpnI |
D GFP forward | CGCGGTACCGACCTGACACAACGCAGTTC | KpnI |
E GFP forward | CGCGGTACCTCACCTTTAAAATCTTAGCCCATT | KpnI |
PhupS GFP reverse 1 | CGCGAATTCGGGCTAGGTGTTTTTGTATTGT | EcoRI |
PhupS GFP reverse 2 | CGCCAATTGGGGCTAGGTGTTTTTGTATTGT | MunI |
PprbcL GFP forward | CGCGGTACCATCGGGCAAGGATTCT | KpnI |
PprbcL GFP reverse | CGCGCAATTGATTTTATCCTTCCCTGAAAT | MunI |
CONFIRMATION | ||
pSUN202 seq primer forward | CAAGTAGCGAAGCGAGCAG | - |
pSUN202 seq primer reverse | TGGGACAACTCCAGTGAAAA | - |
LUCIFERASE | ||
CLONING | ||
A lux forward | CATCTCGTAGGCCCGGTAAT | - |
B lux forward | GCGCGAATTCTCAATCCCCTAAATTG | EcoRI |
C lux forward | GCGCGAATTCTAAAATTCTAGGGGGAAATTG | EcoRI |
D lux forward | GCGCGAATTCGACCTGACACAACGCAGTTC | EcoRI |
E lux forward | GCGCGAATTCACCTTTAAAATCTTAGCCCATT | EcoRI |
PhupS lux reverse | GCGCGGTACCGGGCTAGGTGTTTTTGTATTGT | KpnI |
Pprbc lux forward | GCGCCAATTGCATCGGGCAAGGATTCT | MunI |
Pprbc lux reverse | GCGCGGTACCGGCTTGATACCCAGACTTGC | KpnI |
CONFIRMATION | ||
pLR1 seq forward | AATACCGCACAGATGCGTAA | - |
pLR1 seq reverse | CCAATTAGCGAATAGAGCTT | - |
EMSA | ||
GS forward | GCGCGAATTCCCAATACCTAATACTTAATCCTC | - |
GS reverse | GCGCTCTAGATAACAAAGTTAGGTCTTAA | - |