Figure 3

Optimization of GFP fluorescence measurements. The curves demonstrate GFP intensity within a culture of transformed Nostoc punctiforme ATCC 29133. The cultures have been transformed with a self replicative vector, pSUN202, where truncated versions of the hupSL promoter have been fused to gfp (constructs A to E). Dilutions of the cultures, ranging from 3–30 μg Chl a/ml, have been plotted against the intensity (%). All dilutions have been measured in triplicates and the total fluorescence in the sample is 100%.