Table 1 Oligonucleotide primers used in this study
Name | 5'-3' sequence | Description | Restriction site |
|---|---|---|---|
HS34 | GCTGACTCATAAATATCTGACTG | pigA, primer extension oligo | Â |
HS36 | GCGAAAATAGCTCGGCTGATCTC | smaI, primer extension oligo | Â |
HS60 | GTCTATATCGGCATCTGTTCC | carA, primer extension oligo | Â |
KML | CCAGTAAGTTTTCCAGTAGGTGG | F primer for KmR gene of miniTn5 Km1 | Â |
KMR | CCGAGCTTGGTACCCAGTC | R primer for KmR gene of miniTn5 Km1 | Â |
NW225 | GACCACACGTCGACTAGTGCNNNNNNNNNACTG | Random primed PCR primer 1 | Â |
NW226 | GACCACACGTCGACTAGTGCNNNNNNNNNATGAC | Random primed PCR primer 2 | Â |
NW227 | GACCACACGTCGACTAGTGCNNNNNNNNNGTCTC | Random primed PCR primer 3 | Â |
NW244 | CGTCTGCCAGGTGCTATTGGTTATG | pstSCAB region sequencing primer | Â |
NW245 | GGATAACGAAGTGAACAGCAAC | pstSCAB region sequencing primer | Â |
NW246 | GCATCCTGGCCGAGCATACAGAAG | pstSCAB region sequencing primer | Â |
NW247 | GCGACGCATGCGGATAAGCTCTG | pstSCAB region sequencing primer | Â |
NW250 | CATTACTGCGATGCACAATCAG | phoU sequencing primer | Â |
NW251 | GTGACGATTGATGAAGCTTGTG | phoU sequencing primer | Â |
OTG124 | ATCAGAGAATTCTACTAATTGGAGTCATTACCG | F primer for pTG27, smaI promoter construct | Eco RI |
OTG125 | ATCAGAAAGCTTAGTCTATCATTATAGCGTTCC | R primer for pTG27, smaI promoter construct | Hin dIII |
PF42 | GCATAAGCTTCCATCACTACTCC | R primer for pTA14, rap promoter construct | Hin dIII |
PF43 | GTAAGAATTCGCGATGTTCAGAAAC | F primer for pTA14, rap promoter construct | Eco RI |
PF154 | GATGAATTCAGGAGGACAGGGATGGCAAGACGTATTTTG | F primer for pTA74, PhoB expression construction | Eco RI |
PF155 | TCTAAGCTTCAGTAACGCGTCGAG | R primer for pTA74, PhoB expression construction | Hin dIII |
PF180 | TTTGAATTCGTTAGTTTGGGAGATTTTC | F primer for sequencing phoR | Eco RI |
PF182 | TTTAAGCTTGCTGCGGGACGC | R primer for sequencing phoR | Hin dIII |
PHORL | GCGTTAGTTTGGGAGATTTTC | F phoR primer | Â |
PHORR | CTCCCAAACTAACGCTGTC | R phoR primer | Â |
PST1 | CAGCGTCTGCCAGGTGC | pstS sequencing primer | Â |
PST2 | GTCCACGTTGCTGAG | pstA sequencing primer | Â |
PST3 | CCAGCTTTACCCAGAGCAACATG | pstB sequencing primer | Â |
PST4 | CAGAGTGTAGTTTGCAGG | pstS sequencing primer | Â |
PST5 | CGAGCAACAGCCAGTAG | pstA sequencing primer | Â |
PSTSLN | CAACAGGATAAAGGTAGTGGAGG | pstS sequencing primer | Â |
PSTSRN | CTGCACGGTCTTGGTCG | pstS sequencing primer | Â |
T3 | CGCGCAATTAACCCTCACTAAAG | pBluescript II KS+ sequencing primer | Â |
T7 | GCGCGTAATACGACTCACTATAG | pBluescript II KS+ sequencing primer | Â |