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Table 2 Promoter sequences for jag, gidB, parA and parB genes of M. bovis BCG (Mb) and M. smegmatis (Ms)

From: par genes in Mycobacterium bovis and Mycobacterium smegmatisare arranged in an operon transcribed from "SigGC" promoters

Promoter

-35

Spacer

-10

Spacer

TSS§

Group

P1 jag (Mb)

aaaCGT

16

CAgCCG

03

A

D

P2 jag (Mb)

aGCgGc

18

CGGCCC

11

G

D

P1 gidB (Mb)

TcGAgA

19

aAacAT

04

C

A

P2 gidB (Mb)

cGCaGT

18

CGgCtG

13

A

D

P parA (Mb)

TGtgaT

21

tGtCAG

04

G

D

P1 parB (Mb)

gctACA

17

TAcgAT

12

G

A

P2 parB (Mb)

TGCgCa

20

CACCtC

12

G

D

P jag (Ms)

cGCCGa

18

CGGGAG

10

G

D

P gidB (Ms)

TGCgGc

14

CGggAG

07

G

D

P parA (Ms)

TtCCtT

18

CtCCCG

10

A

D

P parB (Ms)

TcGaCg

16

CGagCG

04

G

D

  1. (†) Length of the spacer between the -35 and -10 hexamers. (‡) Length of the spacer between the -10 hexamer and TSS. (§) Transcription start site (TSS) determined by primer extension. Consensus nucleotides are shown with capital letters.