Mutational and transcriptional analyses of an avian pathogenic Escherichia coli ColV plasmid

BMC Microbiology

Table 4 Primers used in RT-PCR studies.

Name	Description	Sequence	Predicted amplicon size
gapA F	Glyceraldehyde 3-phosphate dehydrogenase A (reference)	CAT CGT TTC CAA CGC TTC CT	84
gapA R		ACC TTC GAT GAT GCC GAA GTT
sitA F	Iron/manganese transport gene	TAC GAT CCG GCA AAT GCA CAA ACC	130
sitA R		TGG TGA CCA TCC ATC GCT GAT TCT
iutA F	Aerobactin receptor gene	TCT GAT AAG AGC GTG GTG GCG AAT	139
iutA R		AGC ACG TTG AAG TTC ACT CCG GTA
hlyF F	Avian hemolysin gene	AAC TTT GGC GGT TTA GGC ATT CCG	164
hlyF R		TGA CAT ACT GGC AAT GAG CCG TCA
etsC F	Putative ABC transport gene	ATT ACG AAC AGC GAG TGC TGG AGT	183
etsC R		ATA CGT ACT GCA CCA TGC CGG TAA
iss F	Increased serum survival gene	GCC GCT CTG GCA ATG CTT ATT ACA	82
iss R		TCC TTT GGT GTT ACT GCT GTC GGT
iroN F	Salmochelin receptor gene	TTC ACC TGG GAA GAT TAC CAC GCA	109
iroN R		ATA TAT GCG CCT GAA GCG GTT TGC
cvaC F	ColV structural gene	CGG GCA ATT TGT TGC AGG AGG AAT	111
cvaC R		ACC GGA TGG AGA CAT TGC AGG ATT
tsh F	Temperature-sensitive hemagglutinin gene	TAC TGA ACC AGC AGG CGG ACA ATA	106
tsh R		TTT ACC TGC CGC TCA TCA GTC AGT

ISSN: 1471-2180