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Table 1 Primers used for target gene by RT-PCR

From: The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus

Primera

Primer sequencesb

PCR product (bp)

SARS-Nf-4

5'- cgc ggatcc tct gat aat gga ccc ca -3'

1266

SARS-Nr-1269

5'- gc ctgcag tta tgc ctg agt tga atc agc aga -3'

 

229E-Nf-4

5'- cgc ggatcc gct aca gtc aaa tgg gct gat -3'

1167

229E-Nr-1170

5'- ccc gtcgac tta gtt tac ttc atc aat tat -3'

 

OC43-Nf-4

5'- cgc ggatcc tct ttt act cct ggt aag caa -3'

1344

OC43-Nr-1347

5'- ccc aagctt tta tat ttc tga ggt gtc ttc -3'

 

SARS-tNf-961

5'- cgc ggtacc att ggc atg gaa gtc aca -3'

309

SARS-tNr-1269

5'- ccc ctgcag tta tgc ctg agt tga atc agc aga -3'

 

229E-tNf-925

5'- cgc ggatcc gtt tcc aaa gag tca ggc aac -3'

246

229E-tNr-1170

5'- ccc gtcgac tta gtt tac ttc atc aat tat -3'

 

OC43-tNf-985

5'- cgc ggatcc tta gag ttg gcc aaa gtg -3'

363

OC43-tNr-1347

5'- ccc aagctt tta tat ttc tga ggt gtc ttc -3'

 

SARS-tSf-790

5'- cgc ggatcc ctc aag tat gat gaa aat ggt aca atc aca -3'

1251

SARS-tSr-2040

5'- gc ggtacc aga cat agt ata agc cac aat aga -3'

 
  1. a f and r signify forward and reverse primers, respectively.
  2. b underlined sequences indicate restriction sites: Bam HI (ggatcc), Pst I (ctgcag), Sal I (gtcgac), Hin dIII (aagctt), and Kpn I (ggtacc).