Strains
|
BE3471
|
PS2209 gltB (psiQ39)::lacZ (Mu d1-1734)
|
[28]
|
BE3479
|
PS2209 gltB (psiQ32)::lacZ (Mu d1-1734)
|
[28]
|
BE3779
|
PS2209 gltB (psiQ32)::lacZ (Mu d1-1734) lrp-35::Tn10
|
[28]
|
BL21(DE3)
|
F- ompT hsdS
B
(rB-mB-) gal dcm (DE3)
|
Novagen
|
JWD3
|
BE1 (W3110 lrp-201:: Tn10) pJWD2
|
[28]
|
LP1000
|
PS2209 gltB::lacZ transcriptional fusion at λatt with -406 to +246 of the gltBDF operon
|
[14]
|
LP1050
|
LP1000 ΔargR
|
This work
|
LP1060
|
BL21DE3/pLP1060
|
This work
|
LP1070
|
BL21DE3/pLP1070
|
This work
|
LP1080
|
BL21DE3/pLP1080
|
This work
|
LP1270
|
PS2209 gltB::lacZ transcriptional fusion at λatt with -270 to +246 of the gltBDF operon
|
This work
|
LP2002
|
W3110 rph+
|
This work
|
LP2010
|
LP2002 ΔlacZYA
|
This work
|
LP2020
|
LP2010 gltB::lacZ transcriptional fusion at λatt with -406 to +246 of the gltBDF operon
|
This work
|
LP2023
|
LP2020 ΔargR
|
This work
|
PM2003
|
PS2209/pPM2005
|
This work
|
PM2004
|
PS2209/pPM2006
|
This work
|
PS2209
|
W3110 Δlac-169
|
F. C. Neidhardt
|
W3110
|
F- prototroph rph
|
F. C. Neidhardt
|
XL1-Blue
|
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δlac-pro [F' proAB lacIqZΔM15 Tn10]
|
Stratagene
|
Plasmids
|
pBH403
|
pKK232-8 with promoterless lacZ gene inserted upstream of cat gene in a derivative of pBR322
|
[61, 62]
|
pPM2005
|
pBH403 with gltB promoter region cloned from BamHI to SalI
|
This work
|
pPM2006
|
pPM2005 with mutations in the Crp-binding sequence
|
This work
|
PJWD2
|
pTrc 99A (Pharmacia) with the lrp coding sequence cloned into the NcoI site
|
[28]
|
pLP1060
|
pET23b (Novagen) with the argR coding sequence cloned into the NdeI/XhoI sites
|
This work
|
pLP1070
|
pET29a (Novagen) with the crp coding sequence inserted into the NdeI/XhoI sites
|
This work
|
pLP1080
|
pET28A (Novagen) with the himA and himD coding sequences inserted in tandem into the NcoI//BamHI sites
|
This work
|
pGEMT-easy
|
Cloning vector
|
Promega
|
pKO3
|
Suicide vector
|
[54]
|
Primers
|
arg1
|
CGCCAGCAGCGCCGAGGACTGCGAC
| |
arg2
|
CACTAATTATTGAGCTAATTAATACCGCGC
| |
arg3
|
CATATGCGAAGCTCGGCTAAG
| |
arg4
|
CTCGAGTTATTAAAGCTCCTGGTCGAACAGCTC
| |
crp5
|
CCATATGGTGCTTGGCAAACCGC
| |
crp6
|
CCTCGAGTTAACGAGTGCCGTAAACGAC
| |
gltP1
|
CGGGATCCCATAATCACATAAATCACTTTTGCTTATC
| |
gltP2
|
ACGCGTCGACAGCGGATTTCCAACTTATCG
| |
gltcrp12
|
CAGTCAATTAATAAAGAATA TAA CGCTAAAGGCGG TTTCTGTACCAATAAGCTTGCC
| |
gltcrp2
|
GGCAAGCTTATTGGTACAGAAACCGCCTTTAGCGTTATATTCTTTATTAATTGACTG
| |
himA1
|
GGTACCGGCATCATTGAGGGATTGAACCTATGGCGCTTACA
| |
himA2
|
CTCGAGCTCGTCTTTGGGCGAAGC
| |
himD1
|
GGTACCTTAACCGTAAATATTGGCGCGATCGCG
| |
himD2
|
TCATGACCAAGTCAGAATTGATAGAAGAC
| |
lac6
|
GGATACGACGATACCGAAGACAGCTCATG
| |
lac7
|
CGAGCCGGAAGCATAAAGTGTAAAGC
| |
lac8
|
GCTTTACACTTTATGCTTCCGGCTCGGGCGTTCCTTGTCGGGTTATTCG
| |
rph1
|
GCGTCTGATCGCCCGTGCTCTTCGCGC
| |
rph23
|
CGTCGCTACAATGGATTCGATTCCCCC TCGGGC
| |
rph33
|
GCCCGAG GGGGAATCGAATCCATTGTAGCGACG
| |
rph4
|
ACGGCAGGTCCAGGTCGTGATGCTCCG
| |