Table 2 E. coli strains1, plasmids, and primers used in this work
Strain/plasmid/primer | Description | Source or reference |
|---|---|---|
Strains | ||
   BE3471 | PS2209 gltB (psiQ39)::lacZ (Mu d1-1734) | [28] |
   BE3479 | PS2209 gltB (psiQ32)::lacZ (Mu d1-1734) | [28] |
   BE3779 | PS2209 gltB (psiQ32)::lacZ (Mu d1-1734) lrp-35::Tn10 | [28] |
   BL21(DE3) | F- ompT hsdS B (rB-mB-) gal dcm (DE3) | Novagen |
   JWD3 | BE1 (W3110 lrp-201:: Tn10) pJWD2 | [28] |
   LP1000 | PS2209 gltB::lacZ transcriptional fusion at λatt with -406 to +246 of the gltBDF operon | [14] |
   LP1050 | LP1000 ΔargR | This work |
   LP1060 | BL21DE3/pLP1060 | This work |
   LP1070 | BL21DE3/pLP1070 | This work |
   LP1080 | BL21DE3/pLP1080 | This work |
   LP1270 | PS2209 gltB::lacZ transcriptional fusion at λatt with -270 to +246 of the gltBDF operon | This work |
   LP2002 | W3110 rph+ | This work |
   LP2010 | LP2002 ΔlacZYA | This work |
   LP2020 | LP2010 gltB::lacZ transcriptional fusion at λatt with -406 to +246 of the gltBDF operon | This work |
   LP2023 | LP2020 ΔargR | This work |
   PM2003 | PS2209/pPM2005 | This work |
   PM2004 | PS2209/pPM2006 | This work |
   PS2209 | W3110 Δlac-169 | F. C. Neidhardt |
   W3110 | F- prototroph rph | F. C. Neidhardt |
   XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δlac-pro [F' proAB lacIqZΔM15 Tn10] | Stratagene |
Plasmids | ||
   pBH403 | pKK232-8 with promoterless lacZ gene inserted upstream of cat gene in a derivative of pBR322 | [61, 62] |
   pPM2005 | pBH403 with gltB promoter region cloned from BamHI to SalI | This work |
   pPM2006 | pPM2005 with mutations in the Crp-binding sequence | This work |
   PJWD2 | pTrc 99A (Pharmacia) with the lrp coding sequence cloned into the NcoI site | [28] |
   pLP1060 | pET23b (Novagen) with the argR coding sequence cloned into the NdeI/XhoI sites | This work |
   pLP1070 | pET29a (Novagen) with the crp coding sequence inserted into the NdeI/XhoI sites | This work |
   pLP1080 | pET28A (Novagen) with the himA and himD coding sequences inserted in tandem into the NcoI//BamHI sites | This work |
   pGEMT-easy | Cloning vector | Promega |
   pKO3 | Suicide vector | [54] |
Primers | ||
   arg1 | CGCCAGCAGCGCCGAGGACTGCGAC |  |
   arg2 | CACTAATTATTGAGCTAATTAATACCGCGC |  |
   arg3 | CATATGCGAAGCTCGGCTAAG |  |
   arg4 | CTCGAGTTATTAAAGCTCCTGGTCGAACAGCTC |  |
   crp5 | CCATATGGTGCTTGGCAAACCGC |  |
   crp6 | CCTCGAGTTAACGAGTGCCGTAAACGAC |  |
   gltP1 | CGGGATCCCATAATCACATAAATCACTTTTGCTTATC |  |
   gltP2 | ACGCGTCGACAGCGGATTTCCAACTTATCG |  |
   gltcrp12 | CAGTCAATTAATAAAGAATA TAA CGCTAAAGGCGG TTTCTGTACCAATAAGCTTGCC |  |
   gltcrp2 | GGCAAGCTTATTGGTACAGAAACCGCCTTTAGCGTTATATTCTTTATTAATTGACTG |  |
   himA1 | GGTACCGGCATCATTGAGGGATTGAACCTATGGCGCTTACA |  |
   himA2 | CTCGAGCTCGTCTTTGGGCGAAGC |  |
   himD1 | GGTACCTTAACCGTAAATATTGGCGCGATCGCG |  |
   himD2 | TCATGACCAAGTCAGAATTGATAGAAGAC |  |
   lac6 | GGATACGACGATACCGAAGACAGCTCATG |  |
   lac7 | CGAGCCGGAAGCATAAAGTGTAAAGC |  |
   lac8 | GCTTTACACTTTATGCTTCCGGCTCGGGCGTTCCTTGTCGGGTTATTCG |  |
   rph1 | GCGTCTGATCGCCCGTGCTCTTCGCGC |  |
   rph23 | CGTCGCTACAATGGATTCGATTCCCCC TCGGGC |  |
   rph33 | GCCCGAG GGGGAATCGAATCCATTGTAGCGACG |  |
   rph4 | ACGGCAGGTCCAGGTCGTGATGCTCCG |  |